Description
Yeast Extract Agar – Premium Medium for Microbial Plate Counting
Product Overview
Yeast Extract Agar is a highly nutritious medium specifically designed for microbial plate counting in water. This medium supports the growth of a variety of bacteria, making it the preferred choice for microbial analysis and water quality testing.
Key Components
| Ingredient | gm/liter |
|---|---|
| Enzymatic digested casein | 6.0 |
| Yeast extract | 3.0 |
| Agar | 15.0 |
Final pH: 7.2 ± 0.2 (at 25°C)
You can adjust or supplement this formulation based on specific performance standards.
Instructions for Use
To prepare Yeast Extract Agar, suspend 24.0 g of the medium in one liter of distilled or deionized water. Mix thoroughly and heat while stirring until completely dissolved. Dispense the solution into flasks and sterilize at 121°C (15 psi) for 15 minutes. This method ensures reliability and accuracy for microbial plate counting.
Principle and Explanation
The key components of Yeast Extract Agar play essential roles in supporting bacterial growth:
- Enzymatic digested casein provides essential amino acids and complex nitrogen sources, promoting healthy microbial development.
- Yeast extract is rich in B-vitamin complexes, ensuring growth for various bacteria.
- Glucose serves as an energy source, enhancing growth conditions.
- Agar acts as a solidifying agent, providing a stable environment for colony isolation.
Appearance Characteristics
- Dehydrated medium: Appears as a free-flowing yellow powder.
- Prepared medium: Appears as a transparent yellow solid, suitable for laboratory use.
Precautions
This medium is for laboratory use only. Do not use dehydrated media that are past their expiration date, show clumping, or have color changes to ensure result accuracy.
Storage Conditions and Shelf Life
Store Yeast Extract Agar in its original packaging, sealed, at 5-30°C. The shelf life of dehydrated medium is three years, while prepared medium should be stored at 2-8°C, protected from light, to maintain optimal stability.
Quality Control
To effectively control quality, inoculate the prepared medium and incubate at 36 ± 1°C for 24-48 hours. This process ensures the reliability of results in microbial plate counting.
Why Choose Yeast Extract Agar?
Yeast Extract Agar excels in reliable and efficient microbial plate counting. Its balanced formulation not only meets stringent standards but also supports diverse microbial growth. Therefore, it is invaluable in water quality assessment and various microbiological applications.
Visit our [Food Hygiene Detection Series] for more related products.
For more information on microbial culture media, check out the [Wikipedia page on Yeast Extract Agar]
High-quality Yeast Extract Agar ideal for microbial plate counting in water testing labs
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