Mueller-Hinton Broth (MHB) for Antimicrobial Susceptibility Testing | Nutrient-Rich Culture Medium

Additional information

Product No

Babio-6231

Specification

250g/500g

Product Use

Used for the cultivation of microorganisms and for sensitivity testing.

Description

Mueller-Hinton Broth (MHB) is a premium nutrient-rich medium widely used in microbiology laboratories for antimicrobial susceptibility testing and the cultivation of various microorganisms. With a balanced formulation, MHB supports the accurate evaluation of antibiotic efficacy, making it an essential tool for researchers, clinical labs, and educational institutions.

Key Features:

  • Nutrient Composition: Contains:
    • Beef Extract (3.0 g/L): Provides essential nitrogen and carbon sources.
    • Soluble Starch (1.5 g/L): Acts as a protective colloid against toxic substances and serves as an energy source through hydrolysis.
    • Acid Hydrolyzate of Casein (17.5 g/L): Supplies vital amino acids, nitrogen, and sulfur.
  • pH Level: Final pH of 7.4 ± 0.2 at 25°C ensures optimal growth conditions for a wide range of bacteria.
  • Low Thymine and Thymidine Content: Ideal for broth dilution antimicrobial susceptibility testing, allowing for precise assessment of antimicrobial agents.

Preparation Instructions:

  1. Suspend 22.0 g of Mueller-Hinton Broth in 1 liter of distilled or deionized water.
  2. Mix thoroughly and heat with frequent agitation until dissolved.
  3. Sterilize in an autoclave at 121°C (15 lbs) for 15 minutes.

Appearance:

  • Dehydrated Medium: Free-flowing yellowish powder.
  • Prepared Medium: Yellowish transparent liquid.

Storage Conditions:

  • Shelf Life: Dehydrated medium: 3 years from the date of manufacture. Prepared medium: Store at 2-8°C away from direct light.
  • Storage Instructions: Keep tightly capped in the original container at 5-30°C. Do not use if past shelf life, caking, or color variation occurs.

Quality Control:

Cultural characteristics should be assessed after incubation at 35-37°C for 18-24 hours to ensure proper functionality and performance.

Applications:

  • Antimicrobial Susceptibility Testing: Widely recognized for use in determining the effectiveness of antibiotics against bacterial pathogens.
  • Microbial Cultivation: Suitable for the general cultivation of a variety of bacteria in laboratory settings.
Microorganism Strains Number Inoculum (CFU) Growth Remarks
Escherichia coli ATCC 25922 10-100 Luxuriant Turbid
Pseudomonas aeruginosa ATCC 27853 10-100 Luxuriant Turbid
Staphylococcus Aureus ATCC 25923 10-100 Luxuriant Turbid
Bacillus subtilis ATCC 6633 10-100 Luxuriant Turbid
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Approximate Formula:

Ingredients gm./liter
Beef extract 3.0
Soluble starch 1.5
Acid hydrolyzate of Casein 17.5
Final pH 7.4±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Additional information

Product No

Babio-6231

Specification

250g/500g

Product Use

Used for the cultivation of microorganisms and for sensitivity testing.

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