1/2 MS Medium (no agar or sucrose) for Plant Tissue Culture

Additional information

Product No

1/2MS Medium

Specification

500g

Product Use

For plant tissue culture

Description

Introducing the 1/2MS Medium no agar and sucrose, a high-quality formulation specifically designed for plant tissue culture applications. With a shelf life of three years, this medium supports robust plant growth and development. It contains a balanced mix of inorganic salts and essential nutrients, making it ideal for various plant species.

Key Ingredients and Composition

Our 1/2MS Medium is composed of critical nutrients that enhance plant tissue culture:

  • Potassium Nitrate (950 mg/L): Essential for plant growth, promoting healthy shoot development.
  • Ammonium Nitrate (825 mg/L): Provides a nitrogen source, crucial for protein synthesis.
  • Potassium Phosphate Monobasic (85 mg/L): Aids in energy transfer within plant cells.
  • Magnesium Sulfate (185 mg/L): Important for chlorophyll synthesis and enzyme activation.
  • Calcium Chloride (220 mg/L): Supports cell wall structure and stability.
  • Trace Elements: Includes Potassium Iodide, Boric Acid, Manganese Sulfate, Zinc Sulfate, Sodium Molybdate, Cupric Sulfate, and Cobalt Chloride to ensure comprehensive nutrient availability.

Benefits of 1/2MS Medium

Using the 1/2MS Medium no agar and sucrose offers numerous benefits for researchers and plant biologists:

  1. Optimized Growth Conditions: The carefully balanced nutrient composition ensures optimal conditions for plant tissue culture, promoting cell division and differentiation.
  2. Versatile Application: Suitable for a wide range of plant species, from agricultural crops to ornamental plants.
  3. Easy Preparation: Simply dissolve 2.47g of the medium in 1000ml of distilled water. Sterilize at 116℃ under high pressure for 30 minutes, and adjust the pH with dilute acid or alkali as needed.

Application and Usage

When preparing the 1/2MS Medium, ensure you follow the recommended procedures for best results. After sterilization, a small amount of precipitate may occur, which is normal and does not affect the medium’s efficacy. This medium is an all-inorganic salt formulation that has proven successful in supporting tissue culture experiments.

Conclusion

For anyone involved in plant tissue culture, the 1/2MS Medium stands out as an essential product. Its scientifically formulated nutrient profile, combined with ease of preparation, makes it a preferred choice for researchers aiming to achieve successful plant growth in vitro. Enhance your plant tissue culture projects today with our premium 1/2MS —a reliable solution for your laboratory needs. visit our Conventional Medium Series for more related products

Potassium Nitrate:950
Ammonium Nitrate:825
Potassium Phosphate Monobasic:85
Magnesium Sulfate:185
Calcium Chloride:220
Potassium lodide:0.83
Boric Acid:6.2
Manganese Sulfate:22.3
Zinc Sulfate:8.6
Sodium Molybdate:0.25
Cupric Sulfate:0.025
Cobalt Chloride.6H2O:0.025
Na2EDTA:37.3
Ferrous Sulfate:27.8
Myo-Inositol:100
Glycin:2
Thiamine.HCI:0.1
Pyridoxine.HCI:0.5
Nicotinic Acid:0.5

Additional information

Product No

1/2MS Medium

Specification

500g

Product Use

For plant tissue culture

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