Description
Introducing the 1/2MS Medium no agar and sucrose, a high-quality formulation specifically designed for plant tissue culture applications. With a shelf life of three years, this medium supports robust plant growth and development. It contains a balanced mix of inorganic salts and essential nutrients, making it ideal for various plant species.
Key Ingredients and Composition
Our 1/2MS Medium is composed of critical nutrients that enhance plant tissue culture:
- Potassium Nitrate (950 mg/L): Essential for plant growth, promoting healthy shoot development.
- Ammonium Nitrate (825 mg/L): Provides a nitrogen source, crucial for protein synthesis.
- Potassium Phosphate Monobasic (85 mg/L): Aids in energy transfer within plant cells.
- Magnesium Sulfate (185 mg/L): Important for chlorophyll synthesis and enzyme activation.
- Calcium Chloride (220 mg/L): Supports cell wall structure and stability.
- Trace Elements: Includes Potassium Iodide, Boric Acid, Manganese Sulfate, Zinc Sulfate, Sodium Molybdate, Cupric Sulfate, and Cobalt Chloride to ensure comprehensive nutrient availability.
Benefits of 1/2MS Medium
Using the 1/2MS Medium no agar and sucrose offers numerous benefits for researchers and plant biologists:
- Optimized Growth Conditions: The carefully balanced nutrient composition ensures optimal conditions for plant tissue culture, promoting cell division and differentiation.
- Versatile Application: Suitable for a wide range of plant species, from agricultural crops to ornamental plants.
- Easy Preparation: Simply dissolve 2.47g of the medium in 1000ml of distilled water. Sterilize at 116℃ under high pressure for 30 minutes, and adjust the pH with dilute acid or alkali as needed.
Application and Usage
When preparing the 1/2MS Medium, ensure you follow the recommended procedures for best results. After sterilization, a small amount of precipitate may occur, which is normal and does not affect the medium’s efficacy. This medium is an all-inorganic salt formulation that has proven successful in supporting tissue culture experiments.
Conclusion
For anyone involved in plant tissue culture, the 1/2MS Medium stands out as an essential product. Its scientifically formulated nutrient profile, combined with ease of preparation, makes it a preferred choice for researchers aiming to achieve successful plant growth in vitro. Enhance your plant tissue culture projects today with our premium 1/2MS —a reliable solution for your laboratory needs. visit our Conventional Medium Series for more related products
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