Description
DPD Medium for Plant Tissue Culture
Product Overview
DPD Medium is a specially designed formulation aimed at optimizing plant tissue culture applications. This product is available in two packaging options: 250g and 500g, with a shelf life of three years, ensuring reliability and consistency in experiments.
Key Ingredients and Benefits
The unique composition of DPD Medium includes balanced nutrients essential for plant tissue growth and development. Specifically, each liter of DPD Medium contains the following key components:
- Ammonium Nitrate: 270 mg/L
- Potassium Sulfate: 1480 mg/L
- Magnesium Sulfate: 340 mg/L
- Calcium Chloride: 569.9 mg/L
- Dihydrogen Potassium Phosphate: 80 mg/L
- Ferrous Sulfate: 27.79 mg/L
- EDTA Disodium Salt: 37.3 mg/L
- Manganese Sulfate: 5 mg/L
- Sodium Molybdate: 0.1 mg/L
- Boric Acid: 2 mg/L
- Zinc Sulfate: 2 mg/L
- Copper Sulfate: 0.015 mg/L
- Cobalt Chloride: 0.01 mg/L
- Potassium Iodide: 0.25 mg/L
- Nicotinic Acid: 4 mg/L
- Pyridoxine Hydrochloride: 0.7 mg/L
- Thiamine Hydrochloride: 4 mg/L
- Myo-Inositol: 100 mg/L
- Folic Acid: 0.4 mg/L
- Glycine: 1.4 mg/L
- Biotin: 0.04 mg/L
- Sucrose: 20000 mg/L
- Mannitol: 54651 mg/L
- 2,4-Dichlorophenoxyacetic Acid (2,4-D): 1 mg/L
- Cytokinin (Kinetin): 0.5 mg/L
Moreover, the pH is optimized to 5.8, ensuring that nutrients are bioavailable to plant cells, thus promoting efficient growth.
Preparation Instructions
To prepare DPD Medium, first, dissolve 77.6g of the powder in 1000ml of distilled water. Next, sterilize the solution at 116°C for 30 minutes. Additionally, it is crucial to adjust the pH to 5.8 before use. It is important to note that this inorganic salt formulation may produce some precipitate after sterilization.
Applications and Uses
DPD Medium is particularly suitable for various plant tissue culture techniques. For instance, it supports callus formation, shoot regeneration, and root induction. Furthermore, both researchers and horticulturists favor DPD Medium, due to its effective support in tissue culture, leading to successful propagation and conservation of various plant species.
Research and Development Advantages
Additionally, this medium is especially useful in plant genetics, breeding programs, and research on genetically modified organisms (GMOs). With its comprehensive nutrient composition, DPD Medium significantly enhances the efficiency of plant tissue culture procedures.
Conclusion
In summary, DPD provides the necessary foundation for successful plant tissue culture applications. Its carefully balanced formulation, straightforward preparation process, and reliable performance make it the preferred choice for researchers and cultivators alike. By choosing DPD , you are investing in a proven product that supports exceptional plant growth and research outcomes.
visit our Plant Tissue Medium Series for more related products
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