Description

E Coli Broth Granule: Efficient Detection of Coliforms

E Coli Broth serves as an essential cultivation medium designed specifically for determining and enumerating coliforms, particularly E. coli, in water and various other materials. This product stands out in microbiological analysis, providing reliable results for laboratories aiming to detect fecal contaminants.

Key Ingredients and Their Functions

The formulation of E Coli Broth includes a carefully balanced mix of components that work together to support the growth of coliform bacteria:

• Pancreatic Digest of Casein (20.0 gm/liter): This ingredient acts as a vital nutrient source, enabling robust bacterial growth.
• Sodium Chloride (5.0 gm/liter): This salt maintains osmotic balance within the culture medium.
• Lactose (5.0 gm/liter): The fermentable sugar facilitates the fermentation process, aiding in identifying lactose-fermenting coliforms.
• Bile Salt No.3 (1.5 gm/liter): This component effectively inhibits the growth of gram-positive bacteria, allowing for a more accurate assessment of coliform presence.
• Phosphate Buffers: Dipotassium hydrogen phosphate (4.0 gm/liter) and potassium dihydrogen phosphate (1.5 gm/liter) stabilize the pH during lactose fermentation, ensuring optimal growth conditions.

The final pH of the medium maintains at 6.9±0.2 at 25°C, which is crucial for the activity of coliforms.

Preparation and Usage Instructions

To prepare the medium, suspend 37 grams of E Coli Broth granules in one liter of deionized or distilled water. Mix thoroughly and heat until fully dissolved. After distributing the prepared medium into test tubes with Durham gas collection tubes, you can effectively monitor gas production, a key indicator of coliform presence. Sterilize the medium at 121°C (15 lbs) for 15 minutes to ensure sterility before use.

Principle of Operation

Laboratories primarily employ E Coli Broth to confirm the presence of fecal coliforms in water and food samples. When inoculated with suspected samples, the medium supports the growth of E. coli and other coliforms, providing preliminary evidence through gas production within 24 hours. To enhance accuracy, incubate the broth at 44.5°C, targeting the isolation of E. coli specifically from both water and shellfish.

Visual Appearance

The dehydrated medium appears as a free-flowing yellowish granule, while the prepared medium transforms into a yellowish transparent liquid, making it easy to handle during laboratory procedures.

Storage and Shelf Life

For optimal performance, store E Coli Broth tightly capped in its original container at temperatures between 5-30°C. The dehydrated medium boasts a shelf life of three years from the date of manufacture, while prepared media can remain at 2-8°C away from direct light.

Quality Control Measures

For quality assurance, inoculate the medium and incubate it at 44.5±0.2°C for 24-48 hours. Regular checks ensure the medium’s efficacy, reinforcing its reliability in detecting coliforms.

In summary, E. Coli Broth provides a powerful tool for microbiologists focused on identifying fecal contamination in water and food products. By integrating high-quality ingredients and a straightforward preparation process, this medium supports accurate and efficient coliform detection, meeting the stringent demands of modern testing protocols. Consider using E Coli Broth in your lab’s routine for precise microbiological analysis.

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