Description
Brain Heart Infusion Broth Granules: Optimal Solution for Bacterial Culture
Brain Heart Infusion Broth (BHI) Granules are a specialized medium designed for the bacterial purification culture and enrichment of diverse microorganisms. This nutrient-rich formulation provides essential growth factors and is ideal for laboratories that require reliable microbial growth conditions. BHI Granules are particularly effective for culturing fastidious bacteria, making them an indispensable tool in microbiological research and clinical diagnostics.
Key Ingredients and Their Benefits
The approximate formula for BHI Granules includes:
- Pancreatic Digest of Casein (10.0 g/L): This ingredient serves as a rich source of amino acids and peptides, providing the necessary nutrients for bacterial growth.
- Beef Heart Extract Powder (17.5 g/L): This component enhances the nutritional profile, supporting the growth of a wide range of fastidious organisms.
- Sodium Chloride (5.0 g/L): Sodium chloride maintains osmotic balance and is crucial for the overall stability of the medium.
- Glucose (2.0 g/L): As a primary energy source, glucose supports active metabolism and proliferation of bacteria.
- Disodium Hydrogen Phosphate (2.5 g/L): This buffering agent stabilizes the pH, ensuring optimal growth conditions with a final pH of 7.4 ± 0.2 at 25 °C.
Together, these ingredients create an environment conducive to the growth and enrichment of various bacterial strains.
Directions for Use
To prepare Brain Heart Infusion Broth, suspend 38.5 g of granules per liter of distilled water. It is essential to autoclave the mixture at 121 °C for 15 minutes to ensure sterilization. Following this procedure guarantees that the medium is free from contaminants, allowing for accurate and reliable results in your bacterial cultures.
Applications in Microbiology
Brain Heart Infusion Broth Granules are widely used in various applications, including:
- Clinical Microbiology: Facilitating the growth of pathogens from clinical specimens for identification and susceptibility testing.
- Research Laboratories: Supporting studies involving fastidious microorganisms, such as those found in human flora or environmental samples.
- Quality Control: Ensuring the efficacy of antimicrobial agents by growing specific test organisms.
The versatility of this broth makes it suitable for both routine laboratory procedures and specialized research applications.
Conclusion
In summary, Brain Heart Infusion Broth Granules provide a robust solution for bacterial purification culture and enrichment. With its well-balanced formulation, it supports the growth of a variety of microorganisms under controlled conditions. Whether you are in a clinical or research setting, BHI Granules are an essential component of your microbiological toolkit. Incorporate them into your workflows to enhance your microbial culture outcomes and achieve reliable, reproducible results.
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