Brilliant Green Lactose Bile Broth for Coliform Detection in Food & Water

Description

Brilliant Green Lactose Bile Broth

Brilliant Green Lactose Bile Broth serves as an essential selective medium for detecting coliform bacteria in various samples, such as food, dairy products, water, and wastewater. It plays a vital role in ensuring food safety and maintaining water quality standards.

Composition and Functionality

This medium contains a blend of peptic digest of animal tissue, lactose, oxgall, and brilliant green dye, which inhibits the growth of gram-positive and some gram-negative bacteria. However, it allows coliform bacteria to thrive by fermenting lactose. As a result, gas production occurs, which is easily detectable in Durham tubes, confirming contamination.

Applications in Various Industries

Brilliant Green Lactose Bile Broth proves highly effective in detecting coliforms in food products such as dairy, meat, and produce. Additionally, it is widely used in wastewater treatment facilities, where monitoring coliform contamination is crucial for public health. Thus, its precise formulation ensures reliable detection, even in complex samples, making it an ideal choice for microbiological testing in different industries.

Optimal Conditions and Physical Characteristics

This medium maintains an ideal pH of 7.2 ± 0.2 at 25°C, creating the perfect environment for coliform detection. In its dehydrated state, the medium appears as a yellowish-green powder, which, when prepared, turns into a green transparent liquid.

Preparation Instructions

To prepare the medium:

1. Suspend 40.0g of Brilliant Green Lactose Bile Broth powder in 1 liter of purified water.
2. Stir thoroughly to dissolve the powder completely, slightly warming the mixture if necessary.
3. Dispense the solution into tubes containing Durham tubes.
4. Autoclave the mixture at 121°C for 15 minutes.
5. Cool the broth quickly to preserve its effectiveness.

Principle of Operation

Brilliant Green Lactose Bile Broth works by selectively allowing coliform bacteria to grow while inhibiting non-coliforms. The combination of oxgall and brilliant green dye blocks the growth of gram-positive and some gram-negative bacteria, while coliforms ferment lactose, producing gas. This gas, observable in the Durham tubes, indicates contamination, making the medium highly reliable for detecting coliform bacteria.

Quality Control Guidelines

To ensure accurate results:

• Inoculate and incubate the medium at 36 ± 1°C for 24-48 hours.
• Observe gas formation in the Durham tubes, which confirms the presence of lactose-fermenting coliforms.

Storage and Shelf Life

To maintain the medium’s effectiveness:

• Store the dehydrated powder in a tightly sealed container at 5-30°C.
• The medium remains usable for up to 3 years from the manufacturing date. After preparation, store the broth at 2-8°C, away from direct light, for short-term use.

Important Precautions

• Use this medium strictly for laboratory purposes.
• Do not use the medium if it has exceeded its shelf life, shows signs of caking, or has changed color.

By using Brilliant Green Lactose Bile Broth, laboratories can effectively detect coliform bacteria across a variety of samples, ensuring compliance with food safety and water sanitation standards. Its selective nature, ease of use, and consistent performance make it an indispensable tool in microbiological testing.

Visit our [Food Hygiene Detection Series] for more related products.

Brilliant Green Lactose Bile Broth used to detect coliform organisms in food, dairy, and water samples