1/2 B5 Medium Plant Tissue Culture (Without Agar or Sucrose)

Additional information

Product No

BB8487-4

Specification

250g/500g

Product Use

For plant tissue culture (without agar and sucrose).

Description

Enhance your plant tissue culture experiments with our 1/2 B5 Medium (without agar or sucrose). This high-quality formulation is specifically designed for efficient plant growth and development. Our 1/2 B5 Medium provides essential nutrients, promoting robust tissue regeneration while excluding agar and sucrose, making it ideal for various plant tissue culture applications.

Key Ingredients and Benefits

This specialized medium includes:

  • Sodium Phosphate Monobasic (75 mg): A vital buffer that maintains the pH level.
  • Calcium Chloride Anhydrous (56.6 mg): Essential for cell wall structure and stability.
  • Potassium Nitrate (1250 mg): Delivers necessary potassium and nitrogen for growth.
  • Ammonium Sulfate (67 mg): Supplies a nitrogen source, enhancing protein synthesis.
  • Magnesium Sulfate Anhydrous (61 mg): Supports chlorophyll production and enzyme function.
  • Na2EDTA (37.3 mg): A chelating agent that improves nutrient availability.

In addition to these core components, our medium contains micronutrients such as Iron (Ferrous Sulfate), Manganese, Zinc, Boron, and others, ensuring a complete nutrient profile for optimal plant tissue growth.

Preparation Instructions

To prepare the 1/2 B5 Medium, follow these simple steps:

  1. Measure 1.7 grams of the medium.
  2. Dissolve it in 1000 mL of distilled water.
  3. Sterilize the solution using 121°C high-pressure sterilization for 15 minutes.
  4. Allow it to cool before use in your plant tissue culture applications.

Versatile Application

Our 1/2 B5 Medium is suitable for a variety of plant species and is widely used in laboratories for research and commercial purposes. It supports various tissue culture techniques, including callus induction, shoot regeneration, and rooting experiments. This flexibility makes it a preferred choice among researchers and horticulturists alike.

Storage and Shelf Life

Store the medium in a cool, dry place, and it has a shelf life of three years. Ensure that the packaging remains sealed until you are ready to use the product, maintaining its efficacy and quality.

Conclusion

In conclusion, our 1/2 B5 Medium (without agar or sucrose) is an essential tool for anyone involved in plant tissue culture. With its carefully balanced nutrients and easy preparation, it supports the growth of healthy plant tissue, facilitating successful research outcomes. Enhance your tissue culture practices today by choosing our high-quality medium! visit our Conventional Medium Series for more related products

Sodium Phosphate Monobasic: 75

Calcium Chloride Anhydrous: 56.6

Potassium Nitrate: 1250

Ammonium Sulfate: 67

Magnesium Sulfate Anhydrous: 61

Na2EDTA: 37.3

Ferrous Sulfate: 27.8

Manganese Sulfate: 10

Zinc Sulfate: 2

Boric Acid: 3

Sodium Molybdate: 0.25

Cupric Sulfate: 0.025

Cobalt Chloride 6H2O: 0.025

Potassium Iodide: 0.75

Thiamine HCl: 10

Pyridoxine HCl: 1

Nicotinic Acid: 1

Myo-Inositol: 100

Additional information

Product No

BB8487-4

Specification

250g/500g

Product Use

For plant tissue culture (without agar and sucrose).

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