Description
Yeast Peptone Dextrose Agar Plate (YPD) – 9cm
The Yeast Peptone Dextrose Agar Plate (YPD) is an essential tool in microbiology, specifically designed for the cultivation and enumeration of yeast and other fungi. This product is packaged in convenient 9 cm disposable sterile plastic plates, making it ideal for laboratories focused on yeast studies, fermentation processes, and quality control in various industries. Each package contains 10 plates, ensuring sufficient supply for extensive testing.
Key Features and Benefits
YPD agar plates provide a nutrient-rich environment that supports the growth of yeast and fungi. The medium contains peptone, which supplies both carbon and nitrogen sources essential for cellular metabolism and growth. Additionally, yeast extract enhances the growth potential by offering B vitamins that are vital for yeast proliferation. Dextrose serves as the primary energy source, while agar acts as a solidifying agent to maintain the integrity of the medium.
Storage and Handling
To ensure optimal performance, it is crucial to store the Yeast Peptone Dextrose Agar Plates at a temperature between 2°C and 25°C. This storage condition preserves the viability and efficacy of the medium. The plates are prepared using the BB5193 formulation, and each plate undergoes low-dose irradiation to guarantee sterility. This process involves a three-layer packaging to protect the agar from contamination and degradation.
Microbial Sensitivity Testing
In microbial sensitivity testing, inoculate the YPD plates with the following control strains and incubate at 23-28°C under aerobic conditions for 48-72 hours. The recovery rate is calculated by comparing the results with the standard SDA agar medium. The following table illustrates the control strains, inoculum size, growth situation, and recovery rates:
| Control Strains | Strain Numbers | Inoculum Size (CFU) | Growth Situation | Results | Recovery Rate |
|---|---|---|---|---|---|
| Yeast | BBATCC18790 | 50-100 | +++ | Smooth, raised white colony | >=70% |
| Candida albicans | BBCMCC(F)98001 | 50-100 | +++ | Smooth, raised white colony | >=70% |
| Aspergillus niger | BBCMCC(F)98003 | 50-100 | +++ | Black spores present | >=70% |
Applications
The Yeast Peptone Dextrose Agar Plate is widely used in various sectors, including food and beverage, pharmaceutical, and biotechnology industries. It is particularly effective in monitoring yeast counts in fermentation processes and ensuring product quality. Researchers and microbiologists benefit from its reliability and simplicity in determining yeast population dynamics.
Conclusion
In summary, the Yeast Peptone Dextrose Agar Plate (YPD) is an indispensable resource for laboratories working with yeasts and fungi. Its carefully formulated composition promotes robust microbial growth, making it an ideal choice for quantitative analysis. With its easy handling, effective storage requirements, and reliable performance in microbial sensitivity testing, YPD plates support the rigorous demands of modern microbiological research. Invest in YPD plates for accurate and efficient yeast cultivation and enumeration in your laboratory today.
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