Description
WPM Medium for Plant Tissue Culture
WPM Medium (without Calcium Nitrate) is specifically designed for optimal plant tissue culture. This versatile formulation is perfect for researchers and horticulturists aiming to cultivate plant tissues efficiently. With a shelf life of three years, this medium provides the essential nutrients required for robust plant growth.
Key Components and Nutrient Profile
The WPM Medium includes a balanced mix of inorganic salts that support plant tissue culture. Key ingredients include:
- Ammonium Salts: 400 mg/L
- Potassium Sulfate: 900 mg/L
- Magnesium Sulfate: 370 mg/L
- Monopotassium Phosphate: 170 mg/L
- Calcium Chloride: 96 mg/L
- Manganese Sulfate: 22.5 mg/L
- Zinc Sulfate: 8.6 mg/L
- Boric Acid: 6.2 mg/L
- Copper Sulfate: 0.25 mg/L
- Sodium Molybdate: 0.25 mg/L
- Ferrous Sulfate: 27.3 mg/L
- EDTA: 37.3 mg/L
- Thiamine Hydrochloride (VB1): 1.0 mg/L
- Pyridoxine Hydrochloride (VB6): 0.5 mg/L
- Niacin (VB3): 0.5 mg/L
- Glycine: 2.0 mg/L
- Myo-Inositol: 100 mg/L
These components work synergistically to promote healthy plant tissue development and enhance growth efficiency.
Preparation and Usage
To prepare the WPM Medium, simply weigh out 2.14 g of the product and add 0.56 g of Calcium Nitrate. Dissolve in 1000 mL of distilled water while heating. After preparation, distribute the solution into appropriate containers and sterilize at 116°C for 30 minutes under high pressure. Adjust the pH as needed to meet specific cultivation requirements.
Benefits of WPM Medium
The WPM Medium is a fully inorganic salt formulation that offers reliable performance for plant tissue culture. Although a small amount of precipitate may occur after sterilization, this does not affect the medium’s efficacy. Researchers and cultivators will appreciate its ease of use and effectiveness in a wide range of plant species.
This product is ideal for those involved in plant tissue culture, including universities, research institutions, and commercial horticulture. With its comprehensive nutrient profile, WPM facilitates successful plant propagation and regeneration, supporting various experimental and production objectives. visit our Plant Tissue Medium Series for more related products
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