Description
VRB-MUG Agar: Reliable Detection of E. coli
VRB-MUG Agar is a high-quality microbiological medium specifically designed for the solid plate detection of E. coli in accordance with GB standards. This agar is essential for laboratories focusing on food safety and environmental monitoring, ensuring accurate results in identifying harmful pathogens.
Composition and Technical Data
The effectiveness of VRB-MUG Agar stems from its carefully formulated components, which include:
- Peptic digest of animal tissue: 7.0 g/L
- Yeast extract powder: 3.0 g/L
- Sodium chloride: 5.0 g/L
- Lactose: 10.0 g/L
- Bile salts: 1.5 g/L
- Crystal violet: 0.002 g/L
- Neutral red: 0.03 g/L
- Agar: 15.0 g/L
- MUG (4-methylumbelliferyl-β-D-glucuronide): 0.1 g/L
This medium has a final pH of 7.4 ± 0.1 at 25℃, which is critical for optimal microbial growth. The components work synergistically to provide a nutrient-rich environment conducive to the selective growth of E. coli while inhibiting other organisms.
Easy Preparation and Use
To prepare VRB-MUG Agar, simply suspend 41.6 grams of the medium in 1 liter of distilled water. Boil the solution for 2 minutes to ensure complete dissolution, then allow it to cool to 45-50°C. Notably, there is no need for autoclaving, making the preparation process straightforward and efficient. This ease of use makes VRB-MUG a preferred choice for both seasoned laboratories and those new to microbiological testing.
Applications in Food Safety and Water Quality Testing
VRB-MUG Agar is particularly valuable in the food industry, where it aids in routine testing for E. coli in various food products. By utilizing this medium, food manufacturers can swiftly identify contamination, thereby ensuring compliance with safety regulations and protecting consumer health. Additionally, this agar is instrumental in water quality testing, allowing laboratories to monitor drinking and recreational water sources for harmful bacteria effectively.
The incorporation of MUG allows for rapid detection through fluorescence, simplifying the identification process. When E. coli is present, it metabolizes MUG, resulting in a detectable fluorescence that signals contamination. This feature enhances both the speed and reliability of testing, making VRB-MUG an indispensable tool in microbiological analysis.
Conclusion
In summary, VRB-MUG Agar provides a reliable and efficient means of detecting E. coli, crucial for safeguarding food safety and ensuring water quality. Its robust formulation and straightforward preparation protocol make it an ideal choice for laboratories committed to high standards in microbiological testing. For further enhancement of your laboratory capabilities, visit our [Chromogenic Medium Series] for more related products that complement VRB-MUG . Ensure accuracy and efficiency in your testing processes today with VRB-MUG !
Reviews
There are no reviews yet.