Violet Red Bile Agar for Coliform Detection in Food

Description

Violet Red Bile Agar: Essential for Coliform Detection in Food and Milk

Violet Red Bile Agar is a highly effective microbiological medium specifically formulated for the detection and enumeration of coliforms in various matrices, including milk, food products, and other materials. As food safety becomes increasingly vital in the industry, utilizing reliable testing methods is crucial. This agar medium allows laboratories and food producers to efficiently monitor microbiological quality, ensuring compliance with safety standards.

Technical Specifications

The typical formula for Violet Red Bile Agar includes:

• Peptone (7.0 g/L): Provides essential nutrients for bacterial growth.
• Yeast Extract (3.0 g/L): Enhances the growth of fastidious organisms.
• Sodium Chloride (5.0 g/L): Maintains osmotic balance.
• Lactose (10.0 g/L): Serves as a fermentable carbohydrate source.
• Bile Salts No.3 (1.5 g/L): Inhibits non-coliform organisms, allowing selective growth.
• Crystal Violet (0.002 g/L): Further suppresses unwanted microbial flora.
• Neutral Red (0.03 g/L): A pH indicator that highlights lactose fermentation.
• Agar (15.0 g/L): Solidifying agent to form a stable gel.

The final pH of this medium is 7.4 ± 0.2 at 25℃, creating an optimal environment for coliform bacteria.

Preparation Instructions

To prepare Violet Red Bile Agar, follow these simple steps:

1. Suspend 41.5 g of the medium in one liter of deionized or distilled water.
2. Mix well and heat with frequent agitation.
3. Boil for one minute to ensure complete dissolution of components.
4. Cool to 45-50℃ before pouring into sterile petri dishes.

Important: Do not autoclave this medium, as it may alter its effectiveness.

Why Choose Violet Red Bile Agar?

Using Violet Red Bile Agar enables efficient coliform testing, a critical aspect of food safety and quality assurance. This medium is particularly popular among laboratories conducting food microbiology tests, allowing for accurate results in identifying coliform presence. It supports the rapid detection of potentially harmful bacteria, aiding in the prevention of foodborne illnesses.

Visit our [Food Hygiene Detection Series] for more related products.