Description

Vibrio Chromogenic Medium: Rapid Detection of Vibrio parahaemolyticus

Vibrio Chromogenic Medium is specifically designed for the rapid detection of Vibrio parahaemolyticus in food, seafood, and patient stool samples. This innovative medium provides an effective solution for laboratories focused on ensuring food safety and public health. With high specificity that minimizes false negatives typically associated with TCBS medium, it stands out as an ideal choice for microbiology professionals.

Key Features and Benefits

The unique formulation of Vibrio Chromogenic Medium allows for easy differentiation of various Vibrio species based on colony color. Vibrio parahaemolyticus colonies appear green and large, while Vibrio cholerae colonies are red. Other Vibrio species manifest in colors ranging from red to blue or colorless, typically forming smaller colonies. This immediate visual differentiation facilitates quick identification, which is essential for timely interventions in food safety concerns.

Composition and Preparation

The medium consists of:

• Special Nutrients: 75.0 g/L
• Mixed Chromogenic Reagent: 4.45 g/L
• Agar: 15.0 g/L
• Final pH: 8.6 ± 0.1 at 25℃

To prepare, simply suspend 18.9 g of the medium in 200 mL of distilled water. Heat gently for 2-5 minutes and cool before pouring into sterile Petri dishes. It’s crucial to maintain appropriate storage conditions; prepared plates should stay dry and protected from light for optimal results.

Quality Control and Performance

Quality control tests on standard strains demonstrate excellent performance. For instance, Vibrio parahaemolyticus ATCC17802 shows robust growth with distinct green, large colonies, affirming the medium’s efficacy. In contrast, Staphylococcus aureus exhibits no growth, ensuring a high level of specificity. Consequently, this precision supports compliance with national and international food safety standards.

Limitations and Considerations

While Vibrio Chromogenic Medium excels in detecting Vibrio parahaemolyticus, it does not directly identify Vibrio cholerae. The specificity for Vibrio parahaemolyticus reaches approximately 90%, making it highly accurate but occasionally necessitating complementary tests for definitive identification.

Storage and Shelf Life

To maintain product integrity, store the powder medium in a dark, dry place at 2-8°C. Prepared plates can last for 2-5 days, provided they are shielded from direct light exposure. Be vigilant about the shelf life; any dry powder that has exceeded its expiration date or exhibits caking or color variation should not be used.

Conclusion

In summary, Vibrio Chromogenic Medium serves as a vital tool for laboratories focused on food safety. It enables the rapid and reliable detection of Vibrio parahaemolyticus. Its user-friendly preparation method, combined with robust quality control measures, ensures that microbiologists can conduct efficient analyses with confidence.

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