Description
Urine Orientation Chromogenic Medium (UTI) – Your Diagnostic Solution for Urinary Tract Infections
Introduction to UTI Medium
Urinary tract infections (UTIs) are a common health issue. Accurate diagnosis is crucial for effective treatment. The Urine Orientation Chromogenic Medium (UTI) serves as an innovative solution designed specifically for the identification and differentiation of key microorganisms responsible for UTIs. This medium utilizes advanced chromogenic technologies, facilitating rapid and reliable results in clinical laboratories.
Key Features and Composition
The Urine Orientation Chromogenic Medium features a well-designed formulation that enhances its diagnostic capabilities. Each liter contains:
- Peptic digest of animal tissue: 15.0 gm
- Chromogenic mix: 4.5 gm
- Agar: 15.0 gm
- Final pH: 7.0 ± 0.2 at 25°C
This unique composition creates an optimal environment for microbial growth while preventing the swarming of Proteus species. Additionally, specific chromogenic substrates react with enzymes produced by Enterococcus spp., E. coli, and other coliforms. This reaction allows for distinct colony color formation, making identification straightforward.
Rapid Identification and Differentiation
One of the standout features of the Urine Orientation Chromogenic Medium is its ability to provide clear visual indicators of various pathogens. For instance, the chromogen X-glucoside enables the identification of enterococci through blue colonies. At the same time, the Red-Galactoside substrate detects E. coli, which produces pink colonies. Moreover, the presence of tryptophan acts as an indicator for tryptophan deaminase activity (TDA), resulting in halos around Proteus, Morganella, and Providencia spp. This capability significantly enhances the accuracy of UTI diagnostics.
Preparation and Usage Instructions
To prepare this effective diagnostic medium, follow these simple steps:
- Suspend 34.5 grams of the Urine Orientation Chromogenic Medium in 1 liter of distilled water.
- Mix well and sterilize the solution by autoclaving at 121°C for 15 minutes.
- Cool the medium to 50°C and pour it into sterile Petri dishes.
This straightforward preparation process ensures that your lab can quickly respond to the needs of patients suffering from UTIs, enhancing overall laboratory efficiency.
Storage Conditions and Shelf Life
For optimal performance, it is essential to store the Urine Orientation Chromogenic Medium tightly capped in its original container. Keep it at a temperature between 10-30°C. The dehydrated medium boasts a shelf life of three years from the date of manufacture. Additionally, prepared media can be stored out of direct light for up to two weeks at 2-8°C, making it a practical choice for busy laboratories.
Conclusion
In summary, the Urine Orientation Chromogenic Medium (UTI) is a highly effective tool for the quick and precise identification of UTI pathogens. With its advanced chromogenic technology and ease of preparation, laboratories can enhance their diagnostic capabilities and provide better patient care. Invest in your laboratory’s efficiency with this innovative medium. Ensure accurate results in your fight against urinary tract infections. For more information on optimizing UTI diagnostics and the benefits of using chromogenic media, explore our website or contact our sales team today!
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