Description
Urea Indole Broth: Essential for Enterobacteria Differentiation
Urea Indole Broth is a specialized microbiological medium designed for the differentiation of enterobacteria based on urease and indole production. This broth plays a crucial role in microbial identification, allowing laboratories to accurately classify various enteric bacteria.
Key Features and Composition
The Urea Indole Broth formula includes essential components such as peptone, D(+) glucose, L-tryptophan, sodium chloride, and urea, each contributing to optimal bacterial growth and metabolic activity. Specifically, the high concentration of urea (20.0 g/L) supports urease activity, while L-tryptophan (1.0 g/L) is vital for indole production. The inclusion of phenol red (0.012 g/L) acts as a pH indicator, providing visual cues for urease activity.
With a final pH of 6.8 ± 0.2 at 25°C, Urea Indole Broth ensures ideal conditions for enterobacteria growth. This medium is highly effective for isolating and identifying organisms like Escherichia coli and Proteus species, which exhibit distinct urease and indole reactions.
Preparation Instructions
To prepare Urea Indole Broth, simply suspend 30.01 grams in 1000 mL of distilled water. Make sure to dissolve the medium completely before sterilizing it by filtration—do not autoclave. After filtration, aseptically dispense the broth into sterile tubes for use in your laboratory tests.
Applications
This broth is not only suitable for clinical microbiology but also valuable in research settings where accurate enterobacteria identification is necessary. By utilizing Urea Indole, you can enhance your laboratory’s efficiency in differentiating pathogenic bacteria, thus ensuring precise diagnostics and effective patient management.
In summary, Indole Broth is an indispensable tool for microbiologists focusing on enteric bacteria. By incorporating this medium into your testing protocols, you can achieve reliable results in identifying urease and indole-producing organisms. Elevate your laboratory’s capabilities today with Urea Broth!
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