Description
Urea Agar Base pH 7.2 is an essential microbiological medium designed specifically for differentiating urease-producing organisms, particularly within the Enterobacteriaceae family. This formulation allows researchers to effectively assess urease activity, which is crucial for accurate bacterial identification.
Key Features
This medium includes a well-balanced formula. Each liter contains:
- Peptic Digest of Animal Tissue (1.0 g)
- Sodium Chloride (5.0 g)
- Dextrose (1.0 g)
- Potassium Dihydrogen Phosphate (2.0 g)
- Phenol Red (0.012 g)
- Agar (12.0 g)
These components ensure optimal growth conditions for various microbial cultures. Additionally, the medium maintains a stable pH of 7.2 ± 0.2 at 25℃, creating an ideal environment for urease testing.
Directions for Use
To prepare Urea Agar Base, follow these simple steps:
- Suspend 2.1 g of the medium in 95 mL of deionized or distilled water.
- Mix thoroughly and heat with frequent agitation.
- Sterilize in an autoclave at 121℃ (15 lbs) for 15 minutes.
Before use, liquefy the medium and cool it to 45℃–55℃. Then, add 5 mL of a filter-sterilized 40% urea solution. This process results in slant-agar tubes that are ready for microbial culture.
Applications
Researchers widely use this agar base for clinical and research purposes. It helps differentiate urease-positive bacteria from negative ones. Furthermore, Urea Agar Base pH 7.2 is vital in food safety testing, environmental monitoring, and healthcare diagnostics.
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