Description
Tryptose Soya Broth – A Versatile Culture Medium for Aerobic Bacteria and Fungi
Tryptose Soya Broth (TSB) serves as an essential liquid culture medium for cultivating aerobic bacteria and fungi. Each convenient 7.5ml tube facilitates easy handling and precise sample injection, making it indispensable for microbiological research and clinical diagnostics.
Composition and Benefits
This high-quality medium combines pancreatic digest of casein and soybean meal, providing rich nitrogen sources, vitamins, and growth factors. Additionally, glucose serves as a vital carbon source, while dipotassium phosphate acts as a buffering agent, maintaining pH balance. Moreover, sodium chloride ensures osmotic equilibrium, enhancing microbial growth and stability.
Quality Control and Sensitivity Testing
Researchers rigorously test Tryptose Soya Broth for microbial sensitivity. This medium supports various quality control strains, demonstrating its effectiveness. When incubated at 30-35°C for 18-24 hours, the following strains show significant turbidity, indicating robust microbial proliferation:
| Quality control strain | Strain number | Inoculum amount (CFU) | Growth condition | Result |
|---|---|---|---|---|
| Staphylococcus aureus | CMCC(B)26003 | 10 – 100 | +++ | Turbid |
| Pseudomonas aeruginosa | CMCC(B)10104 | 10 – 100 | +++ | Turbid |
| Bacillus subtilis | CMCC(B)63501 | 10 – 100 | +++ | Turbid |
| Escherichia coli | CMCC(B)44102 | 10 – 100 | +++ | Turbid |
| Salmonella paratyphi B | CMCC(B)50094 | 10 – 100 | ++++ | Turbid |
| Candida albicans | CMCC(F)98001 | 10 – 100 | +++ | Turbid (cultured at 20 – 25°C for 48 – 72 hours) |
| Aspergillus niger | CMCC(F)98003 | 10 – 100 | +++ | Produce mycelium (cultured at 20 – 25°C for 48 – 72 hours) |
| Bacillus subtilis | CMCC(B)63501 | 10 – 100 | +++ | Turbid (cultured at 20 – 25°C for 48 – 72 hours) |
Easy Handling and Packaging
Researchers appreciate the convenient packaging of Tryptose Soya Broth in plastic tubes, which ensures easy storage and retrieval. Each box contains 20 tubes, all securely sealed to maintain sterility and integrity. This format allows quick access during laboratory procedures, making TSB a preferred choice for microbiologists.
Applications in Microbiology
Microbiologists widely use Tryptose Soya Broth in various applications, from clinical microbiology to food safety testing. Its formulation supports a diverse range of microorganisms, making it suitable for isolating and identifying bacteria and fungi in various samples. Researchers and laboratory professionals consistently rely on TSB for its reliability and effectiveness in promoting microbial growth. visit our [Ready-to-use culture medium] for more related products
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