Description
Trypticase Soy Polymyxin Broth Base (10ml) – Ideal for Bacillus cereus Testing
Trypticase-Soy-Polymyxin Broth Base, packaged in convenient 10ml plastic tubes, is specifically designed for the MPN (Most Probable Number) determination of Bacillus cereus. Each tube is independently sealed to ensure leak-proof and contamination-free handling, making it an essential choice for microbiological testing in laboratories.
Key Components and Their Functions
This broth base contains essential nutrients to support the growth of target microorganisms. Trypticase peptone and soy peptone serve as nitrogen sources, providing vitamins and growth factors crucial for microbial development. Additionally, glucose acts as the primary carbon source, promoting vigorous growth. The inclusion of dipotassium phosphate functions as a buffering agent, maintaining pH stability throughout the incubation period. Sodium chloride helps to maintain osmotic balance, ensuring that the microbial cultures thrive without stress.
Polymyxin B, a key feature of this broth, effectively inhibits the growth of unwanted contaminants, allowing for accurate results when assessing Bacillus cereus. This selective pressure makes Trypticase-Soy-Polymyxin Broth Base a reliable medium for distinguishing between target bacteria and potential contaminants.
Microbial Sensitivity Testing
For accurate quality control, the broth supports testing with reference strains. Below is a detailed table showcasing the microbial sensitivity testing results:
| Quality control strain | Strain number | Inoculum amount (CFU) | Reference or counting medium | Method | Quality control result | Other characteristics |
|---|---|---|---|---|---|---|
| Bacillus cereus, Escherichia coli | CMCC(B)63303, ATCC25922 | 10 – 100, 1000 – 5000 | TSA | Semi-quantitative | More than 10 CFU on MYP | Colonies are slightly pink with a light pink precipitation ring around. |
| Bacillus cereus, Escherichia coli | ATCC11778, ATCC25922 | 10 – 100, 1000 – 5000 | TSA | Semi-quantitative | More than 10 CFU on MYP | Colonies are slightly pink with a light pink precipitation ring around. |
| Escherichia coli | ATCC25922 | 1000 – 5000 | TSA | Semi-quantitative | Less than 100 CFU on TSA | Inhibition. |
Advantages of Using Trypticase-Soy-Polymyxin Broth
This broth is an excellent choice for laboratories focused on food safety, clinical diagnostics, and environmental testing. By employing Trypticase-Soy-Polymyxin Broth Base, microbiologists can ensure accurate quantification of Bacillus cereus while minimizing the risk of contamination from other organisms. The robust formulation allows for consistent results across various sample types, including food products and clinical specimens.
Conclusion
In summary, Trypticase-Soy-Polymyxin Broth Base is a critical tool for microbiological testing. Its specialized formulation supports the growth of Bacillus cereus while suppressing contaminants, making it indispensable for accurate MPN determination. Trust this broth to provide reliable results in your microbiological studies, enhancing the overall quality and safety of your testing procedures. visit our [Ready-to-use culture medium] for more related products
Reviews
There are no reviews yet.