Trypticase Soy Broth for Microbial Cultivation

Description

Trypticase (Tryptic) Soy Broth: Your Ideal Microbial Cultivation Medium

Overview

Trypticase (Tryptic) Soy Broth (TSB) is a versatile and essential medium specifically designed for the cultivation of both fastidious and non-fastidious microorganisms. Given its broad applicability, it serves as an invaluable resource in microbiology labs.

Key Ingredients & Composition

Final pH: 7.3 ± 0.2 at 25°C

This combination of ingredients provides a rich nutrient source, thereby ensuring robust growth for a wide range of microorganisms. Consequently, TSB is particularly useful in both clinical and research settings.

Preparation Instructions

1. Dissolve: Begin by suspending 30.0 g of Tryptic Soy Broth in 1 liter of distilled or deionized water.
2. Mix Thoroughly: Stir well and heat with frequent agitation until fully dissolved. This step is crucial for achieving a homogeneous mixture.
3. Sterilize: Next, dispense the mixture into triangle flasks and autoclave at 121°C (15 lbs.) for 15 minutes. This ensures the elimination of any potential contaminants.

Appearance

• Dehydrated Medium: The dehydrated form appears as a free-flowing yellowish powder.
• Prepared Medium: In contrast, the prepared medium takes the form of a yellowish liquid, which is ideal for supporting microbial growth.

Storage Conditions

To maintain its effectiveness, consider the following storage guidelines:

• Dehydrated Medium: Store tightly capped at 5-30°C. Notably, it has a shelf life of 3 years from the date of manufacture.
• Prepared Medium: In addition, store out of direct light at 2-8°C to ensure optimal stability and performance.

Quality Control

For quality assurance, inoculate the broth and incubate at 35±1°C for 18-24 hours. This rigorous testing guarantees that Tryptic Soy Broth meets high performance standards essential for reliable results.

Conclusion

In summary, Trypticase (Tryptic) Soy Broth is a dependable and effective medium for microbial cultivation. Its straightforward preparation process, long shelf life, and high-quality formulation make it an essential choice for laboratories focused on both research and diagnostics. Thus, when you choose Tryptic Soy Broth, you can achieve consistent and optimal results in your microbiological studies.

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