Trypticase Soy Broth Granule for Microorganism Cultivation

Description

Trypticase (Tryptic) Soy Broth Granule for Microorganism Cultivation

Trypticase (Tryptic) Soy Broth Granule serves as a high-performance general-purpose medium used in microbiological testing. Laboratories worldwide rely on this medium for cultivating fastidious and non-fastidious microorganisms from diverse clinical and nonclinical specimens. It provides essential nutrients that promote robust bacterial growth, making it a top choice for microbial research, clinical diagnostics, and pharmaceutical testing. The formulation of Trypticase Soy Broth ensures a stable environment for microorganism proliferation.

Key Ingredients and Formula

This medium’s well-balanced composition supports bacterial growth effectively:

• Pancreatic Digest of Casein (17.0 g/L) offers a rich source of amino acids and complex nitrogenous substances that nourish microorganisms.
• Peptic Digest of Soya Bean (3.0 g/L) delivers additional nutrients, which enhance the growth potential of a broad range of microorganisms.
• Sodium Chloride (5.0 g/L) helps maintain the osmotic balance, thus supporting cell integrity.
• Dipotassium Hydrogen Phosphate (2.5 g/L) acts as a buffer, stabilizing pH levels to create an ideal environment for microbial cultivation.
• Glucose Monohydrate (2.5 g/L) provides a readily available energy source to fuel microbial metabolism.

This precise formulation results in a final pH of 7.3±0.2 at 25°C, which is perfect for a wide array of microbial growth conditions.

Preparation and Usage

To prepare the Trypticase Soy Broth, dissolve 30.0 grams of the medium in one liter of distilled or deionized water. Mix thoroughly while heating with frequent agitation. Next, dispense the medium into sterile flasks, and autoclave at 121°C (15 lbs.) for 15 minutes. This method ensures complete sterilization, making the medium ready for immediate laboratory use.

Principle and Interpretation

The blend of pancreatic digest of casein and peptic digest of soya bean provides the essential nutrients required for bacterial growth. These ingredients supply amino acids and nitrogenous compounds that are critical for the proliferation of fastidious microorganisms. Moreover, the glucose monohydrate serves as a primary energy source, enhancing the metabolic activity of microorganisms. With the inclusion of sodium chloride, osmotic pressure remains balanced, supporting optimal cell function. Furthermore, dipotassium hydrogen phosphate buffers the medium, maintaining a consistent pH for reliable microbial growth.

Appearance and Quality Control

In its dry form, Trypticase Soy Broth Granule appears as a yellowish, free-flowing granule. When mixed and prepared, it transforms into a clear yellow liquid, conducive to microorganism cultivation. For quality control, inoculate the medium with specific test strains and incubate it at 35±1°C for 18-24 hours. If the medium supports expected microbial growth, it confirms its effectiveness and reliability.

Storage and Shelf Life

Store Trypticase Soy Broth Granule in a tightly sealed container at temperatures ranging from 5-30°C to preserve its quality. The dehydrated form remains stable for up to three years from the manufacturing date. For prepared media, keep it at 2-8°C, away from direct light, to maintain its integrity and prevent contamination.

Precautions

Ensure that the medium is used exclusively for laboratory purposes. Before using, inspect the granules for any signs of caking or discoloration, as these indicate a compromised product. Always adhere to storage guidelines to maximize the medium’s effectiveness. visit our [Other Series] for more related products