Description
Trypticase (Tryptic) Soy Broth Granules: Premium Medium for Microbial Cultivation
Product Overview
Trypticase (Tryptic) Soy Broth granules are a highly versatile culture medium suitable for cultivating a wide range of microorganisms, including both fastidious and non-fididious types. This nutrient-rich medium is widely used in clinical and research laboratories to support the growth of various microbes.
Key Ingredients
The typical formulation per liter of medium includes:
- Tryptone (Casein digest): 17.0 g
- Soytone (Soybean digest): 3.0 g
- Sodium chloride: 5.0 g
- Dipotassium phosphate: 2.5 g
- Glucose monohydrate: 2.5 g
Final pH: 7.3 ± 0.2 (at 25°C)
Preparation Instructions
To prepare the medium:
- Suspend 30.0 g of dry powder in 1 liter of distilled or deionized water.
- Mix thoroughly and heat with frequent stirring.
- Dispense into flasks.
- Sterilize at 121°C (15 lbs) for 15 minutes.
Physical Appearance
- Dry Medium: Free-flowing yellow granules.
- Prepared Medium: Yellow liquid.
Storage Conditions
For optimal results, store the medium sealed in its original packaging at a temperature between 5-30°C. The shelf life is 3 years from the date of manufacture. Prepared medium should be kept at 2-8°C and protected from direct light to maintain stability.
Quality Control
To ensure the effectiveness of the medium, it is recommended to incubate at 35±1°C for 18-24 hours. Note that this medium is for laboratory use only. Discard any expired, clumped, or discolored dry medium immediately.
Summary
Overall, Trypticase Soy Broth granules provide a reliable solution for effectively cultivating various microorganisms. Whether handling clinical samples or conducting research, this medium will help you meet your microbial cultivation needs. By using this broth, you can better manage and optimize microbial growth processes, enhancing the accuracy and reliability of your experimental results. It is an essential component of your laboratory resources.
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