Description
Tryptic Soy Agar: The Essential Medium for Microbial Cultivation
Tryptic Soy Agar (TSA) is a well-established culture medium, renowned for its ability to support the growth of both nonfastidious and fastidious microorganisms. This nutrient-rich medium is vital for microbiological research and clinical applications, providing an optimal environment for microbial isolation and cultivation.
Approximate Formula:
| Ingredients | gm/liter |
|---|---|
| Pancreatic digest of casein | 15.0 |
| Enzymatic Digest of Soybean Meal | 5.0 |
| Sodium Chloride | 5.0 |
| Agar | 15.0 |
| Final pH | 7.3±0.2 at 25℃ |
*Adjusted and/or supplemented as required to meet performance criteria.
Preparation Instructions
To prepare Tryptic Soy Agar, follow these simple steps:
- Suspend 40.0g of Tryptic Soy Agar in one liter of deionized or distilled water.
- Mix thoroughly and heat with frequent agitation until boiling.
- Boil the solution for 1 minute.
- Dispense into appropriate tubes or flasks.
- Sterilize in the autoclave at 121°C (15 lbs.) for 15 minutes.
This preparation ensures that the medium is free from contamination and ready for use in microbial studies.
Appearance and Storage
The dehydrated medium appears as a free-flowing yellowish powder. Once prepared, it takes on a yellowish transparent gel form, indicating successful preparation.
Store Tryptic Soy Agar tightly capped in its original container at temperatures between 5–30°C. The dehydrated medium has a shelf life of three years from the manufacturing date. Prepared agar can be stored at 2–8°C, away from direct light, ensuring its efficacy for future experiments.
Quality Control
To ensure the reliability of Tryptic Soy, perform quality control by inoculating the medium and incubating it at 36±1°C for 18-24 hours. This step confirms the medium’s performance in promoting microbial growth.
Conclusion
In summary, Tryptic Soy is an indispensable tool in laboratories, offering a reliable and nutrient-dense medium for microbial studies. Its rich formula supports a wide array of organisms, making it ideal for various applications in microbiology. Optimize your laboratory results with Tryptic Soy and experience its effectiveness in your microbial cultivation needs!
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