Description
Triple Sugar Iron Agar (TSI) – Comprehensive Product Overview
Product Description
Triple Sugar Iron Agar (TSI) is a specialized culture medium crucial for differentiating gram-negative enteric bacilli based on carbohydrate fermentation and hydrogen sulfide production. This versatile medium is widely used in microbiology laboratories for the identification of various pathogens.
Key Features
| Feature | Description |
|---|---|
| Carbohydrates | Contains dextrose, lactose, and sucrose for effective fermentation assessment. |
| Hydrogen Sulfide Detection | Includes ferrous ammonium sulfate to identify hydrogen sulfide production (black precipitate). |
| pH Indicator | Phenol red changes from red to yellow, indicating acid production during fermentation. |
Composition
| Ingredient | Amount (g/L) |
|---|---|
| Pancreatic Digest of Casein | 15.0 |
| Dextrose | 1.0 |
| Beef Extract | 3.0 |
| Sucrose | 10.0 |
| Yeast Extract | 3.0 |
| Phenol Red | 0.025 |
| Sodium Chloride | 5.0 |
| Ferrous Sulfate | 0.2 |
| Proteose Peptone | 5.0 |
| Sodium Thiosulfate | 0.3 |
| Lactose | 10.0 |
| Agar | 12.0 |
Final pH: 7.4 ± 0.2 at 25°C
Preparation Instructions
- Suspend 64.5 g of TSI powder in 1 L of purified water.
- Mix thoroughly and heat with frequent agitation until dissolved.
- Autoclave at 121°C for 15 minutes.
- Cool in a slanted position to form deep butts.
Applications
- Microbial Identification: Essential for identifying enteric bacteria, including Salmonella and Shigella.
- Clinical Diagnostics: Aids in the diagnosis of gastrointestinal infections.
- Research Use: Valuable for studies involving microbial metabolism and fermentation processes.
Storage Conditions
- Dehydrated Medium: Store tightly capped at 5-30°C; shelf life is 3 years from manufacturing.
- Prepared Medium: Store at 2-8°C, away from direct light.
Quality Control
Inoculate and incubate at 36±1°C for 18-24 hours to ensure consistent quality and performance.
Conclusion
Triple Sugar Iron Agar is a vital tool in microbiology for the differentiation of gram-negative enteric bacilli. Its precise formulation enables accurate testing for carbohydrate fermentation and hydrogen sulfide production, making it indispensable for clinical diagnostics and research.
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