TPY Agar Medium for Microbial Culture – Nutrient-Rich, High-Performance Laboratory Growth Medium

Description

Elevate Your Microbial Research with TPY Agar Medium!

TPY Agar Medium is a high-quality, nutrient-rich formulation designed specifically for the isolation and cultivation of a wide variety of microorganisms. This versatile laboratory medium is an essential tool for microbiological testing, food safety analysis, and clinical diagnostics.

Key Features:

• Nutrient-Rich Formula: Our TPY Agar Medium contains a balanced blend of casein hydrolysate (10.0 g) and peptone from soy (5.0 g), providing essential amino acids and complex nitrogen sources that promote robust microbial growth.
• Yeast Extract Boost: With yeast powder (2.0 g) included, this medium supplies the Vitamin B complex necessary for optimal organism development.
• Energy Source: The addition of glucose (5.0 g) serves as a primary energy source, ensuring that your microorganisms thrive during incubation.
• pH Stability: Dipotassium phosphate (2.0 g) acts as a buffering agent to maintain a stable pH of 6.5 ± 0.1 at 25°C, creating ideal growth conditions.
• Trace Elements: A carefully selected blend of minerals including magnesium chloride (0.5 g), zinc sulfate (0.25 g), calcium chloride (0.15 g), and ferric chloride (0.000001 g) provide essential trace elements that support cellular functions and metabolic processes.
• Solidifying Agent: Agar (20.0 g) is included to solidify the medium, allowing for easy handling and isolation of colonies.
• Growth Factor: Tween 80 (1.0 g) enhances the growth of fastidious microorganisms, ensuring comprehensive microbial culture.

Preparation Instructions:

1. Dissolve: Suspend 46.4 g of TPY Agar Medium in 1 liter of distilled or deionized water.
2. Heat: Mix well and heat with frequent agitation until completely dissolved.
3. Dispense: Dispense the mixture into triangle flasks.
4. Sterilize: Autoclave at 121°C (15 psi) for 15-20 minutes to ensure sterility.

Appearance:

The dehydrated TPY Agar Medium is a free-flowing yellowish powder, and upon preparation, it forms a yellowish gel that is visually appealing and easy to work with.

Storage Conditions:

• Store the dehydrated medium tightly capped in the original container at 5-30°C.
• Ensure that the product is used within its 3-year shelf life from the date of manufacturing.
• Prepared medium should be stored out of direct light at 2-8°C for maximum stability.

Quality Control Protocol:

To ensure the highest quality, perform the following quality control:

• Inoculation: Inoculate the medium with a suitable microorganism.
• Incubation: Incubate at 35±1°C for 18-24 hours.
• Verification: Confirm growth and ensure that the medium supports the expected microbial species effectively.

Applications:

TPY Agar Medium is widely used in:

• Microbiological Research: Ideal for studying a variety of microbial species in laboratory settings.
• Food Safety Testing: Assessing the presence of pathogens and spoilage organisms in food products.
• Clinical Diagnostics: Cultivating clinical isolates for identification and susceptibility testing.

Conclusion:

TPY Agar Medium is your reliable partner for successful microbial culture. With its comprehensive nutrient profile and rigorous quality control measures, you can trust TPY Agar to deliver consistent and reproducible results in your laboratory work.

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