Description
Thioglycollate Medium Tube (7.5ml): An Essential for Anaerobic Culture
Overview
The Thioglycollate Medium Tube is a vital tool for researchers and laboratory professionals working with anaerobic culture applications. Each tube contains 7.5ml of specialized medium specifically designed to support the growth of anaerobic bacteria, making it particularly useful in pharmaceutical microbiology. Packaged in a box of 20 tubes, this product offers convenience and safety, with individual packaging that effectively prevents contamination and leakage.
Key Components and Their Functions
The formulation of thioglycollate medium includes several essential components that work together to create an optimal growth environment:
Thioglycollate
Thioglycollate serves as a reducing agent, neutralizing any mercury-based preservatives. This action helps maintain the oxidation-reduction potential of the medium, which is crucial for promoting the growth of anaerobic bacteria and ensuring accurate results in microbiological testing.
Agar Concentration
We have slightly increased the agar concentration from 0.5g to 0.75g. This enhancement stabilizes the reducing environment while maintaining adequate anaerobic conditions.
Redox Indicator
Resazurin acts as an excellent oxidation-reduction indicator. With a concentration of 0.001g, it balances redox potential indication and minimizes any inhibitory effects on bacterial growth.
Carbohydrate Source
Glucose provides a reliable carbon source, contributing to the medium’s oxidation-reduction potential and reducing thioglycollate’s inhibitory impact on Clostridium species.
Yeast Extract and Enzymatic Hydrolysate
The medium contains 0.5% yeast extract and 1.5% pancreatic digest of casein, significantly enhancing bacterial growth. This optimal blend creates a rich environment conducive to the proliferation of various bacterial strains.
L-Cysteine
L-Cysteine promotes the growth of Clostridium sporogenes, a critical strain for testing anaerobic growth capabilities.
Sodium Chloride
Sodium chloride maintains balanced osmotic pressure, which is essential for the overall health of microbial cultures.
Quality Control and Testing
The Thioglycollate Medium Tube allows for effective quality control testing of microbial strains. After inoculating with specified strains, results are readily observed after 24-72 hours of aerobic incubation at 30-35°C.
| Quality control strain | Strain number | Inoculum (CFU) | Growth condition | Result |
|---|---|---|---|---|
| Staphylococcus aureus | BB CMCC(B) 26003 | 10 – 100 | +++ | Granular growth in test tube. |
| Pseudomonas aeruginosa | BB CMCC(B) 10104 | 10 – 100 | +++ | Growth on the surface layer of test tube with turbidity. |
| Bacillus subtilis | BB CMCC(B) 63501 | 10 – 100 | +++ | Growth on the surface layer of test tube with turbidity. |
| Clostridium sporogenes | BB CMCC(B) 64941 | 10 – 100 | +++ | Striped growth below the oxidation layer of test tube. |
| Clostridium perfringens | BB ATCC13124 | 10 – 100 | +++ | Striped growth below the oxidation layer of test tube. |
Packaging and Safety
Each Thioglycollate Medium Tube comes packaged in a plastic screw-cap tube designed to prevent leaks and maintain sterility. The independent packaging of each 7.5ml tube ensures the medium’s integrity until use. visit our [Ready-to-use culture medium] for more related products
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