Tetrathionate Broth Base Granule

Description

Tetrathionate Broth Base Granule for Salmonella Enrichment

Tetrathionate Broth Base Granule provides an excellent medium for the selective enrichment of Salmonella, widely used in food safety testing and water testing laboratories. This product supports the growth of Salmonella spp. while inhibiting the growth of competing organisms. By using the proper additives like brilliant green and iodine, the broth creates the ideal environment for accurate Salmonella detection.

Key Ingredients and Their Functions

This broth’s balanced composition effectively promotes Salmonella growth:

• Peptic digest of animal tissue (9.0 g/1050mL): Supplies essential nutrients to enhance bacterial proliferation.
• Beef powder (4.5 g/1050mL): Contributes vitamins and growth factors to support the selective medium.
• Sodium chloride (2.7 g/1050mL): Maintains the osmotic balance needed for bacteria to thrive.
• Calcium carbonate (40.5 g/1050mL): Buffers the pH for a stable environment.
• Bile salts (5.0 g/1050mL): Inhibits non-Salmonella species, allowing selective growth of the target bacteria.
• Sodium thiosulfate (50.0 g/1050mL): Neutralizes harmful by-products and facilitates the selective growth of Salmonella.

These ingredients work together to create a medium that enhances pathogen detection and enables accurate Salmonella enrichment.

Preparation and Usage Instructions

To prepare the Tetrathionate Broth Base, follow these simple steps:

1. Suspend 93.55g of the product in 1050ml of water.
2. Boil the solution briefly to fully dissolve the granules.
3. Add 20ml of 20% iodine solution and 10ml of 0.1% brilliant green solution. This step is crucial for optimizing the selective properties.
4. Use the broth immediately after preparation for the best results.

By following these steps, you ensure that the broth is properly prepared, which leads to more effective Salmonella testing.

Applications in Food and Water Testing

Tetrathionate Broth Base is essential in detecting Salmonella in various industries, particularly food safety and environmental water testing. Its selective nature makes it highly useful in identifying contamination in processed foods, raw materials, and even ready-to-eat products. Water quality laboratories also rely on this broth to detect Salmonella contamination in water systems, rivers, and lakes.

The ability to accurately detect Salmonella is critical in preventing outbreaks and ensuring regulatory compliance. With Tetrathionate Broth Base, laboratories can efficiently carry out pathogen testing, protecting both consumers and public health.

Quality and Storage Guidelines

Store Tetrathionate Broth Base in a cool, dry place to maintain its effectiveness. Proper storage guarantees the broth will perform optimally during testing and provide reliable, consistent results.

Conclusion

Tetrathionate Broth Base Granule is a critical tool for Salmonella enrichment in both food safety testing and water testing. Its carefully designed formula, enhanced by the addition of brilliant green and iodine, ensures selective Salmonella growth while inhibiting other bacteria. Easy to prepare and highly effective, this broth allows laboratories to detect Salmonella spp. with precision. Regular use of this broth helps meet pathogen detection standards, ensuring that public health remains protected.

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