Description
Soybean-Casein Digest Agar: The Ideal Medium for Microbial Cultivation
Soybean-Casein Digest Agar serves as a highly effective medium for isolating and cultivating both nonfastidious and fastidious microorganisms. This nutrient-rich formulation supports diverse microbial growth, making it a staple in microbiology laboratories.
Key Features
- Nutritional Composition: The unique blend of enzymatic digests of casein and soybean meal provides essential organic nitrogen, including amino acids and longer-chained peptides. These nutrients play a crucial role in promoting optimal microbial development.
- Optimal pH Balance: With a final pH of 7.3 ± 0.2 at 25°C, Soybean-Casein Digest Agar maintains a stable environment that fosters robust microbial activity.
- Versatile Usage: Researchers often use this medium to support a wide variety of microorganisms. However, it does not suit anaerobic bacteria.
Preparation Instructions
To prepare Soybean-Casein Digest Agar, follow these steps:
- Suspend 40.0 g of the medium in one liter of deionized or distilled water.
- Mix well and heat with frequent agitation.
- Boil for one minute, then dispense into tubes or flasks.
- Sterilize in the autoclave at 121°C (15 lbs.) for 15 minutes.
This straightforward process makes the medium convenient for laboratory use.
Storage Guidelines
For optimal performance, store the dehydrated medium in a tightly capped original container at temperatures ranging from 5-30°C. Ensure the product is used before its shelf life of three years from the manufacturing date. Additionally, keep prepared medium out of direct light at 2-8°C to maintain quality.
Quality Control Measures
Researchers should carefully observe cultural characteristics after incubating the medium at 25 ± 1°C for 48 hours. This practice ensures the medium meets established performance criteria and effectively supports microbial growth.
Approximate Formula for Soybean-Casein Digest
Ingredients (per liter):
- Enzymatic Digest of Casein: 15.0 g
- Enzymatic Digest of Soybean Meal: 5.0 g
- Sodium Chloride: 5.0 g
- Agar: 15.0 g
Final pH: 7.3 ± 0.2 at 25°C
Note: Researchers may adjust or supplement the formula as needed to meet specific performance criteria.
In summary, Soybean-Casein Digest Agar stands out as a highly nutritious and reliable medium for microbial research. Its balanced formulation allows for effective microbial isolation and cultivation, making it a trusted choice for researchers seeking consistent results in their experiments.
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