Soybean Casein Digest Agar Granule

Description

Soybean Casein Digest Agar Granule for Microorganism Cultivation

Soybean Casein Digest Agar Granule is a versatile medium designed for the isolation and cultivation of both nonfastidious and fastidious microorganisms. Commonly used in clinical, pharmaceutical, and environmental laboratories, this medium provides the necessary nutrients to support microbial growth, making it suitable for general microbiological testing. However, it is not recommended for the cultivation of anaerobic microorganisms.

Key Ingredients and Nutritional Benefits

The Soybean Casein Digest Agar formula contains several important ingredients that contribute to its efficacy in microbial growth. Enzymatic digest of casein (15.0 g/L) and enzymatic digest of soybean meal (5.0 g/L) provide organic nitrogen sources, such as amino acids and peptides, which are essential for the growth of a wide variety of microorganisms. Sodium chloride (5.0 g/L) helps maintain osmotic balance, ensuring that the cells remain in a stable environment. Finally, agar (15.0 g/L) serves as the solidifying agent, allowing for easy colony observation and microbial isolation.

Preparation and Usage Instructions

To prepare Soybean Casein Digest Agar Granule, suspend 40.0 grams of the medium in one liter of deionized or distilled water. Mix thoroughly and heat with frequent stirring. Boil for one minute, then dispense the medium into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes under 15 lbs. of pressure. This ensures sterility and makes the medium suitable for laboratory use.

Principle and Application

This medium is known for its ability to support the growth of both fastidious and nonfastidious microorganisms, making it highly effective in various testing environments. Casein and soy peptones in the formulation provide essential nutrients that promote bacterial proliferation. These components make the medium ideal for isolating a wide range of bacteria, from clinical pathogens to environmental isolates. The medium’s sodium chloride ensures that the osmotic pressure remains balanced, preventing cellular stress, while agar allows for solid culture preparation.

Appearance and Quality Control

In its dehydrated form, Soybean Casein Digest Agar Granule appears as free-flowing yellowish granules. Once prepared, it forms a yellowish transparent gel, which is ideal for easy colony visibility and growth differentiation. To ensure its effectiveness, quality control tests must be performed. Incubate at 25±1°C for 48 hours and observe the resulting cultural characteristics to confirm the medium’s performance.

Storage and Shelf Life

For optimal performance, store Soybean Casein Digest Agar Granule in its tightly sealed, original container at 5-30°C. When stored correctly, the dehydrated medium has a shelf life of three years from the date of manufacture. Prepared media should be kept at 2-8°C, out of direct light, to preserve its integrity.

Precautions

This medium is intended for laboratory use only. Be sure to check the expiration date before use, as outdated, caked, or discolored granules should not be used for microbial testing. Proper handling and storage are crucial to maintaining the medium’s effectiveness in supporting microbial growth. visit our [Other Series] for more related products