Selenite Cystine Broth for Selective Salmonella Culture

Description

Selenite Cystine Broth (SC) – Selective Enrichment Medium for Salmonella Detection

Selenite Cystine Broth (SC) is an essential culture medium specifically designed for the selective enrichment of Salmonella spp. This broth plays a critical role in microbiological testing, adhering to both GB and SN standards. With its unique formulation, SC offers optimal conditions for isolating Salmonella from various samples, ensuring accurate results.

Key Components

Each liter of Selenite Cystine Broth contains:

• Peptic Digest of Animal Tissue (5.0 g): Provides essential nutrients for microbial growth.
• Lactose (4.0 g): Serves as a fermentable carbohydrate source.
• Sodium Selenite (4.0 g): Functions as a selective agent against competing flora.
• Disodium Hydrogen Phosphate (5.5 g) and Potassium Dihydrogen Phosphate (4.5 g): Maintain the pH and provide buffering capacity.
• L-Cystine (0.01 g): Supports metabolic processes in Salmonella.

The final pH of the medium is adjusted to 7.0 ± 0.1 at 25℃, creating an environment conducive for Salmonella growth.

Preparation Instructions

To prepare Selenite Cystine Broth, simply suspend 23.0 g of the medium in 1 L of distilled water. After mixing, aseptically dispense the solution into sterile flasks or tubes. Importantly, this medium is ready for use on the day of preparation without the need for autoclaving.

Quality Control

When testing for Salmonella, inoculate the broth with 10-100 CFU and incubate at 36±1℃ for 18-24 hours. Following incubation, expect noticeable turbidity, indicating successful growth of Salmonella. This characteristic muddy appearance confirms the effectiveness of the Selenite Cystine Broth in selective enrichment.

Conclusion

Selenite Cystine stands out as a reliable choice for laboratories focusing on Salmonella detection. By utilizing this specialized medium, you enhance your ability to isolate and identify Salmonella efficiently, contributing to food safety and public health. Experience the benefits of using SC for all your microbiological testing needs!

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