Description

Potato Dextrose Agar (USP) – Premium Medium for Yeast and Mold Isolation

Potato Dextrose Agar (USP) is a highly effective medium specifically formulated for the isolation and enumeration of yeasts and molds from dairy and various food products. This versatile agar supports robust fungal growth, making it an essential tool in food microbiology and mycology research.

Key Features:

• Optimal Composition: Each liter contains 6.0 g of potato infusion, 20.0 g of dextrose, and 20.0 g of agar, creating a nutrient-rich environment conducive to yeast and mold proliferation.
• Ideal pH Level: The final pH of 5.6 ± 0.2 at 25°C effectively inhibits bacterial growth while promoting fungal activity.
• Easy Preparation: Simply suspend 46.0 g of the dehydrated medium in one liter of distilled water, heat, autoclave, and pour into Petri dishes for immediate use.

Applications:

Potato Dextrose Agar is recommended by leading authorities, including the APHA and FDA, for conducting plate counts of yeasts and molds. Additionally, it is suitable for stimulating sporulation and maintaining stock cultures of specific dermatophytes. Researchers often rely on this medium for differentiating dermatophyte varieties based on pigment production.

Appearance and Storage:

The dehydrated medium appears as a cream to yellow homogeneous powder. Once prepared, it transforms into a yellowish to milky white, semitransparent gel, perfect for laboratory analyses. Store the dehydrated medium tightly capped in its original container at temperatures between 5-30°C to ensure maximum shelf life of three years.

Quality Control:

For optimal results, inoculate and incubate your samples at 28 ± 1°C for 48-96 hours. This procedure allows for accurate monitoring and ensures the reliability of your findings in yeast and mold studies.

Overall, Potato Dextrose Agar (USP) is indispensable for anyone involved in food safety and microbiological research. Its efficacy in cultivating fungi makes it a top choice among laboratories worldwide. Whether you’re testing dairy products or other food items, this medium delivers consistent and reliable results.

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