Description
Plate Count Agar is an essential medium for the cultivation of bacteria and the enumeration of microorganisms in various samples, including water, sewage, and feces. Formulated to meet the highest standards, our agar is compliant with guidelines from APHA, PHLS, and ISO for accurate microbial counts in food safety testing and environmental monitoring.
Key Ingredients:
- Pancreatic Digest of Casein (5.0 g/l): Provides vital amino acids and nitrogenous compounds essential for robust bacterial growth.
- Yeast Extract (2.5 g/l): Enriches the medium with Vitamin B complex, promoting the growth of diverse microbial populations.
- Dextrose (1.0 g/l): Supplies necessary sugars for enhanced microbial proliferation.
- Agar (15.0 g/l): Acts as a solidifying agent, forming a stable gel for reliable colony counting.
Preparation Instructions:
To prepare the medium, suspend 23.5 g of Plate Count Agar in 1 liter of deionized or distilled water. Heat with frequent agitation until fully dissolved, then boil for 1 minute. Dispense into sterile tubes or flasks and sterilize in an autoclave at 121°C (15 lbs.) for 15 minutes.
Principle and Use:
Plate Count Agar serves as the go-to medium for the plate count of microorganisms in food and other materials. Following the APHA pour plate method, diluted samples are added to Petri plates before sterile molten agar is introduced, ensuring even mixing. This method provides higher recovery rates compared to surface inoculation, making it ideal for applications in sterile environments and laboratories.
Physical Appearance:
Our dehydrated Plate Count Agar appears as a free-flowing yellowish powder, which reconstitutes into a transparent yellowish gel upon preparation.
Storage and Shelf Life:
Store tightly capped in the original container at 5-30°C. The dehydrated medium boasts a shelf life of 3 years from the manufacturing date, while the prepared medium can be stored away from light at 2-8°C.
Quality Control:
Cultural characteristics should be evaluated after incubation at 35-37°C for 18-48 hours, ensuring optimal performance for microbial analysis.
Why Choose Plate Count Agar?
Our Plate Count Agar is not just a laboratory tool; it’s a critical component in ensuring food safety and monitoring environmental quality. By facilitating accurate bacterial enumeration, this medium supports your efforts in achieving the highest standards of public health and safety.
| Microorganism | Strains
Number |
Inoculum
(CFU) |
Growth | Recovery | Remarks |
| Pseudomonas aeruginosa | ATCC 9027 | 20-200 | luxuriant | ≥70% | Colorless transparent colonies |
| Escherichia coli | ATCC 25922 | 20-200 | luxuriant | ≥70% | Colorless transparent
large colonies |
| Bacillus subtilis | ATCC 6633 | 20-200 | luxuriant | ≥70% | White irregular colonies |
| Bacillus cereus | ATCC 11778 | 20-200 | luxuriant | ≥70% | White irregular colonies |
| Staphylococcus Aureus | ATCC 6538 | 20-200 | luxuriant | ≥70% | Yellowish colonies |
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