Description
PDP Tube for Pseudomonas Aeruginosa Pyocyanin Determination
Product Overview
Introducing the PDP Tube, specifically designed for the determination of pyocyanin produced by Pseudomonas aeruginosa (commonly known as green pus bacillus). Each box contains 20 tubes of 13ml capacity, providing a reliable solution for microbiological testing in laboratories. This product is essential for researchers and professionals working in microbiology, clinical diagnostics, and environmental testing.
Key Features
The PDP Tube is a glass tube with a screw cap, ensuring a secure seal to prevent leakage and contamination. The formulation includes essential components such as peptone, magnesium chloride, potassium sulfate, agar, glycerol, and distilled water, which provide the necessary nutrients for Pseudomonas aeruginosa growth and pyocyanin production.
Testing Principle
The testing principle is straightforward. Peptone and glycerol serve as carbon and nitrogen sources, while magnesium chloride and potassium sulfate enhance the production of pyocyanin and fluorescein. The agar acts as a solidifying agent, creating an optimal environment for bacterial growth.
When conducting the test, you inoculate the PDP Tube with a suspected colony, incubate it at 36±1℃ for 24±2 hours, and then extract pyocyanin using chloroform. This method provides clear results, indicating whether Pseudomonas aeruginosa is producing pyocyanin through color changes during the acidification process.
Microbial Sensitivity Testing
Sensitivity Testing Table
| Quality control strain | Strain number | Inoculum amount (CFU) | Reference or counting medium | Method | Quality control result | Other characteristics |
|---|---|---|---|---|---|---|
| Pseudomonas aeruginosa | CMCC(B)10104 | / | / | Qualitative | Pyocyanin positive | / |
| Escherichia coli | CMCC(B)44102 | / | / | Qualitative | Pyocyanin negative | / |
Usage Instructions
To use the PDP Tube effectively:
- Inoculate: Use an inoculating needle to streak the suspected colony on the slant surface of the PDP Tube.
- Incubate: Place the tube in a suitable incubator at 36±1℃ for 24 hours.
- Extract: Add 3-5 mL of chloroform to the tube, shake well, and allow it to stand for 15-20 minutes.
- Test for Color Change: Transfer the chloroform layer to a new tube, add about 1 mL of 1 mol/L hydrochloric acid, shake, and observe for a pink to red color change, indicating a positive result for pyocyanin.
Quality Control
For optimal results, quality control is crucial. Inoculate with control strains of Pseudomonas aeruginosa (CMCC(B)10104), and Escherichia coli (CMCC(B)44102), and conduct tests under aerobic conditions at 35°C. This step ensures the reliability of your testing results.
Safety and Precautions
- Inspect the PDP Tubes before use. Do not use any tube that is broken, discolored, or contaminated.
- During the extraction process, ensure you operate in a fume hood due to the hazardous nature of chloroform. Use appropriate personal protective equipment (PPE) to safeguard against exposure.
- Avoid tightly sealing the tubes during incubation to prevent false negatives, as Pseudomonas aeruginosa is an obligate aerobe.
Conclusion
The PDP Tube for Pseudomonas aeruginosa pyocyanin determination is a valuable tool for microbiological assessments. With its reliable design, precise formulations, and straightforward testing methods, it meets the needs of professionals in various fields. Make the PDP Tube a part of your laboratory toolkit to ensure accurate and effective microbial testing. visit our [Ready-to-use culture medium] for more related products
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