Description

NT Medium: Essential Plant Tissue Culture Medium

NT Medium is a specialized formulation designed for optimal plant tissue culture, ideal for researchers and horticulturists aiming to achieve successful plant regeneration. This nutrient-rich medium offers a balanced combination of inorganic and organic components, providing essential support for plant growth.

Inorganic Composition

NT Medium contains a carefully curated selection of inorganic salts, which play a vital role in plant nutrition:

• Ammonium Nitrate (NH4NO3): 825 mg/L
• Potassium Nitrate (KNO3): 950 mg/L
• Magnesium Sulfate (MgSO4·7H2O): 1233 mg/L
• Calcium Chloride (CaCl2·2H2O): 220 mg/L
• Monopotassium Phosphate (KH2PO4): 680 mg/L
• EDTA (C10H14N2Na2O8·2H2O): 37.3 mg/L
• Ferrous Sulfate (FeSO4·7H2O): 27.8 mg/L
• Manganese Sulfate (MnSO4·4H2O): 22.3 mg/L
• Zinc Sulfate (ZnSO4·7H2O): 8.6 mg/L
• Boric Acid (H3BO3): 6.2 mg/L
• Potassium Iodide (KI): 0.83 mg/L
• Sodium Molybdate (Na2MoO4·2H2O): 0.25 mg/L
• Copper Sulfate (CuSO4·5H2O): 0.025 mg/L
• Cobalt Sulfate (CoSO4·7H2O): 0.030 mg/L

This balanced blend of minerals ensures that your plant cultures receive the nutrients necessary for robust growth.

Organic Components

In addition to its inorganic makeup, NT Medium includes vital organic compounds:

• Vitamin B1 (Thiamine Hydrochloride): 1.0 mg/L
• Myo-Inositol: 100.0 mg/L
• Naphthaleneacetic Acid (NAA): 3.0 mg/L
• Sucrose: 10,000 mg/L
• Mannitol: 127,519 mg/L

These organic components are crucial for enhancing cellular metabolism and promoting vigorous growth in plant tissues.

Preparation Instructions

To prepare NT Medium, measure 141.63 grams of the product and dissolve it in 1,000 mL of distilled water. Sterilize the solution at 116°C for 20 minutes using high-pressure sterilization techniques. After sterilization, a small amount of precipitate may occur, which is typical for this type of plant tissue culture medium.

Applications and Benefits

NT Medium is perfect for a wide variety of plant tissue culture applications, including the propagation of rare and endangered species, research on plant development, and genetic modification studies. Its specific formulation supports a high growth rate and successful tissue regeneration.

With a pH value of 5.8, NT Medium provides an optimal environment for plant cells to thrive, ensuring that researchers can achieve their desired outcomes efficiently. This product is an indispensable tool for anyone involved in plant biotechnology and research. visit our Plant Tissue Medium Series for more related products