Description

NLN Medium for Plant Tissue Culture

NLN Medium, a specialized nutrient solution designed for plant tissue culture, is an essential component for successful in vitro plant growth. This medium, available in 250g and 500g packages, is ideal for researchers and horticulturists seeking a reliable formulation to support their plant propagation efforts. With a shelf life of three years, NLN Medium provides the stability needed for long-term use.

Composition Breakdown

The NLN Medium is meticulously formulated with a range of essential nutrients, ensuring optimal growth conditions for plant cells. Below is a detailed list of its components:

• CoCl2·6H2O (Cobalt Chloride): 0.025 mg/L
• CuSO4 (Copper Sulfate): 0.025 mg/L
• Sodium EDTA (Ethylenediaminetetraacetic Acid): 36.70 mg/L
• H3BO3 (Boric Acid): 10.00 mg/L
• MnSO4·H2O (Manganese Sulfate): 18.95 mg/L
• Na2MoO4 (Sodium Molybdate): 0.250 mg/L
• ZnSO4 (Zinc Sulfate): 10.00 mg/L
• KNO3 (Potassium Nitrate): 125.0 mg/L
• K2HPO4 (Dibasic Potassium Phosphate): 125.0 mg/L
• MgSO4 (Magnesium Sulfate): 61.00 mg/L
• Biotin: 0.05 mg/L
• Folic Acid: 0.5 mg/L
• L-Glutamine: 800.0 mg/L
• L-Glutathione (Reduced): 30.00 mg/L
• Glycine: 2.00 mg/L
• Inositol: 100.0 mg/L
• Niacin: 5.0 mg/L
• Pyridoxine Hydrochloride: 0.5 mg/L
• L-Serine: 100.0 mg/L
• Thiamine Hydrochloride: 0.5 mg/L

This well-balanced composition supports cell division and differentiation, making NLN Medium a top choice in the field of plant tissue culture.

Preparation Instructions

To prepare NLN Medium, simply weigh 1.43g of the medium, and add 0.5g of calcium nitrate. Heat this mixture to dissolve in 1000ml of distilled water. Sterilize by autoclaving at 116°C for 30 minutes. It’s important to note that a small amount of precipitate may form after sterilization, which is normal for this inorganic salt-based medium.

Key Benefits

NLN is specifically designed to enhance plant tissue culture techniques. Its unique formulation is perfect for promoting the growth of various plant species in a controlled environment. Whether you are conducting research or engaging in commercial plant propagation, NLN Medium provides the essential nutrients required for successful tissue culture.

Utilizing NLN can significantly improve plant regeneration rates, supporting the growth of healthy, vigorous plants. The absence of calcium nitrate allows for flexibility in formulations, catering to specific experimental needs.

By incorporating NLN into your plant tissue culture practices, you can achieve superior results, making it an indispensable tool for researchers and horticulturists alike. Optimize your plant growth today with NLN —your solution for reliable and effective tissue culture. visit our Plant Tissue Medium Series for more related products