Description
NB Medium serves as an essential growth solution designed specifically for plant tissue culture applications. This high-quality formulation is available in two convenient sizes: 250g and 500g. With a shelf life of three years, NB Medium ensures long-lasting reliability for researchers and horticulturists alike.
Key Ingredients
The unique composition of NB Medium supports optimal plant growth and development. Each ingredient plays a crucial role in nurturing plant tissues, promoting cell division, and facilitating regeneration. Here’s a detailed breakdown of the components per liter:
- Potassium Nitrate (KNO3): 2830 mg
- Ammonium Sulfate ((NH4)2SO4): 463 mg
- Calcium Chloride (CaCl2·2H2O): 166 mg
- Dihydrogen Phosphate (KH2PO4): 400 mg
- Magnesium Sulfate (MgSO4·7H2O): 185 mg
- Boric Acid (H3BO3): 3 mg
- Manganese Sulfate (MnSO4·4H2O): 10 mg
- Zinc Sulfate (ZnSO4·7H2O): 2 mg
- Sodium Molybdate (Na2MoO4·2H2O): 0.25 mg
- Copper Sulfate (CuSO4·5H2O): 0.025 mg
- Cobalt Chloride (CoCl2·6H2O): 0.025 mg
- Potassium Iodide (KI): 0.75 mg
- EDTA (Na2·EDTA): 37.25 mg
- Iron Sulfate (FeSO4·7H2O): 27.85 mg
- Myo-Inositol: 100 mg
- Thiamine Hydrochloride (VB1): 10 mg
- Pyridoxine Hydrochloride (VB6): 1 mg
- Nicotinic Acid (VB5): 1 mg
Preparation Instructions
To prepare NB Medium, weigh out 4.237g of the powder and dissolve it in 1000ml of distilled water. Next, autoclave the solution at 116°C for 30 minutes to ensure sterilization. After autoclaving, a small amount of precipitate may form; this occurrence is normal in inorganic salt formulations.
Application and Benefits
NB Medium proves particularly effective for plant tissue culture due to its all-inorganic salt composition. This formulation provides essential nutrients that support the growth of various plant species. Researchers can utilize NB Medium for micropropagation, somatic embryogenesis, or callus culture, facilitating efficient plant tissue development.
Furthermore, scientists seek out reliable media formulations like NB to achieve consistent results in their experiments. Its balanced nutrient profile promotes healthy growth and makes it a preferred choice in laboratories focused on plant biotechnology and agricultural research.
In addition, the growing interest in sustainable agriculture highlights the need for efficient plant propagation techniques. By using a quality growth medium like NB Medium, researchers can meet the demands of modern plant tissue culture practices. Its finely tuned components ensure optimal results in various experimental settings. visit our Plant Tissue Medium Series for more related products
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