Description
MS Medium for Plant Tissue Culture
The MS Medium (Murashige and Skoog Medium) is a key nutrient medium, widely used for plant tissue culture in labs and research facilities. It provides the essential balance of macronutrients and micronutrients that plants need for growth and development in in vitro conditions. This medium supports a range of applications, including micropropagation, genetic studies, and plant biotechnology.
Preparation and Usage
To prepare the medium, dissolve 41.74 grams of MS powder in 1000 ml of distilled water. Stir the solution thoroughly as it heats to ensure complete dissolution. After that, dispense the mixture into tubes or flasks. Autoclave the medium at 116°C (15 lbs) for 30 minutes to ensure sterility, crucial for successful plant tissue culture.
MS is frequently chosen as the base medium for growing plant cells and tissues. Its formula includes vital nutrients such as nitrogen, potassium, and phosphorus, which promote plant growth. Additionally, it contains vitamins and hormones that stimulate plant tissue differentiation and shoot formation.
Applications in Biotechnology
MS is indispensable in plant biotechnology. It supports the growth of a variety of plant tissues, including explants, roots, and shoots. It is frequently used for the propagation of orchids, tobacco, and cereal crops. Researchers and commercial growers rely on it for micropropagation and plant regeneration.
This medium enables researchers to manipulate plant hormones to control tissue development. Whether you are cultivating rare species or experimenting with plant genes, MS ensures that the tissue receives the nutrients it needs for healthy growth and organ formation.
Key Details
- Weight: 41.74g per liter
- Sterilization: Autoclave at 116°C for 30 minutes
- pH: Adjust to 5.8 before autoclaving
- Storage: Keep in a cool, dry place
This medium works well with supplements like agar and plant growth regulators such as auxins and cytokinins, depending on the specific plants being cultivated.
Why MS Medium?
MS remains the top choice for plant tissue culture, as it provides reliable results across diverse plant species. It facilitates plantlet regeneration and tissue differentiation, making it a trusted solution for both research labs and commercial production. The medium’s composition allows for easy customization, ensuring that it fits various experimental needs. visit our [Plant Tissue Medium Series] for more related products
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