Description
Mold and Yeast Chromogenic Medium: Overview
Introduction
The Mold and Yeast Chromogenic Medium is designed for the identification and differentiation of molds and yeasts. This medium utilizes color reactions that occur when specific organisms grow, allowing microbiologists to easily distinguish between species.
Key Features
The chromogenic medium effectively suppresses bacterial growth while promoting yeast and mold colonies. After incubation at 25-28 °C for 3-5 days, distinct colony colors emerge:
- Candida albicans: Blue-green
- Candida tropicalis: Blue-green
- Candida glabrata: Blue-green
- Candida krusei: Blue-green
- Saccharomyces cerevisiae: Blue-green
- Molds: Cotton-like blue-green appearance
These color indicators simplify initial assessments and further testing.
Ingredients and Preparation
To prepare the medium, mix the following ingredients:
- Peptic digest of animal tissue: 30.0 g/L
- Chromogenic reagent: 1.8 g/L
- Chloramphenicol: 0.1 g/L
- Agar: 15.0 g/L
- Final pH: 6.0 ± 0.2
For preparation, dissolve 9.5 g in 200 mL distilled water, boil briefly, then cool to 45-50 °C before pouring into sterile petri dishes. Use the plates immediately for best results.
Quality Control
Quality control ensures reliable outcomes. When tested with standard strains of yeast and mold, consistent results appear. For example, the following table summarizes the quality control results:
| QC Strains | Strains No. | Growth | Colonies Color |
|---|---|---|---|
| Candida albicans | CMCC98007 | +++ | Blue-green |
| Candida tropicalis | ATCC13803 | +++ | Blue-green |
| Candida krusei | ATCC14243 | +++ | Blue-green |
| Candida glabrata | ATCC15126 | +++ | Blue-green |
| Saccharomyces cerevisiae | CMCC98002 | +++ | Blue-green |
| Mold | CMCC98003 | +++ | Cotton-like Blue-green |
| E. Coli | CMCC44568 | – | – |
Storage and Limitations
Store the powdered medium in a dark, dry place at 2-8 °C. Prepared plates should avoid direct light exposure and be used promptly. Although the medium performs well, rare false positives may occur, highlighting the need for confirmatory tests.
Conclusion
In summary, the Mold and Yeast Chromogenic Medium serves as an essential tool for microbial analysis in laboratories. Its ability to differentiate yeast and mold species enhances efficiency and accuracy in diagnostics. By adopting this medium, laboratories can improve their microbial management practices effectively. For further information about our products, please contact us!
visit our [Chromogenic Medium Series] for more related products
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