Description
MKTTN Broth: ISO 6579-Compliant Selective Enrichment Media for Salmonella Detection
Overview
MKTTN Broth (Müller-Kauffmann Tetrathionate Novobiocin Broth) is a specialized enrichment medium designed for the selective isolation and growth of Salmonella spp. in food, environmental, and clinical samples. Fully compliant with ISO 6579-1:2017 standards, this formulation ensures high sensitivity and specificity in detecting Salmonella, including hard-to-culture strains, making it indispensable for food safety protocols and regulatory compliance.
Key Features
- Dual Selective Action: Contains bile salts (4.78 g/L) and novobiocin (0.04 g/L) to suppress Gram-positive bacteria (e.g., Staphylococcus) and coliforms, while promoting Salmonella growth.
- Tetrathionate Mechanism: Sodium thiosulfate (30.5 g/L) reacts with iodine to form tetrathionate, selectively enriching Salmonella through enzymatic activity (tetrathionate reductase).
- ISO 6579 Validation: Validated for use in food chain microbiology, including raw meat, dairy, eggs, and environmental swabs, aligning with global regulatory requirements.
- Pre-Sterilized Convenience: Dehydrated granules (89.5 g/L) require no autoclaving—simply boil, cool, and add iodine solution for immediate use.
- Extended Stability: Prepared broth remains viable for 4 weeks at 2–8°C, reducing laboratory waste and streamlining workflows.
Applications
- Food Safety Testing: Detect Salmonella in poultry, processed meats, and dairy products to prevent outbreaks and ensure compliance with HACCP protocols.
- Environmental Monitoring: Test water sources, surface swabs, and production facilities for fecal contamination indicators.
- Clinical Diagnostics: Isolate enteric pathogens from stool samples in cases of suspected foodborne illness.
Technical Specifications
- pH Stability: Maintains 8.2 ± 0.2 to optimize bacterial metabolism and inhibitor efficacy.
- Nutrient-Rich Formula: Pancreatic digest of casein (8.6 g/L) and beef extract (4.3 g/L) provide essential carbon, nitrogen, and vitamins for robust Salmonella growth.
- Quality Assurance: Batch-tested for consistency in performance, with strict adherence to ISO 6579-1:2017 guidelines.
Why Choose MKTTN Broth?
- Enhanced Sensitivity: Detects low-level Salmonella contamination even in mixed microbial environments.
- Cost-Effective Workflow: Eliminates autoclaving steps and reduces preparation time by 50% compared to traditional media.
- Versatile Compatibility: Suitable for both manual streaking and automated systems for PCR confirmation.
- Customizable Solutions: Available in bulk quantities (250g to 25kg) with OEM/ODM packaging options for industrial-scale laboratories.
Note: Due to the presence of calcium carbonate, the prepared medium may appear cloudy with suspended particles—this is normal and does not affect performance. For optimal results, mix thoroughly before dispensing into tubes or plates.
Ordering Information
- Packaging: 250g, 500g, or 1kg sterile pouches.
- Storage: Keep unopened containers at 5–30°C in a dry, dark environment.
This product is intended for laboratory use only. Not for diagnostic or therapeutic purposes.
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