Description

mE.Coli Broth (mEC+n) Granule for Selective Enrichment of E. coli O157

Introduction to mE.Coli Broth

Enhance your laboratory’s capabilities with our mE.Coli Broth (mEC+n), specifically designed for the selective enrichment cultivation of E. coli O157. This specialized broth is perfect for microbiological testing, ensuring optimal growth conditions for accurate detection of this pathogenic strain. With a carefully formulated composition, our broth meets the highest performance criteria, making it an essential tool in food safety and clinical diagnostics.

Key Components and Composition

The mE.Coli Broth contains a balanced mixture of nutrients essential for the growth of E. coli O157:

• Pancreatic Digest of Casein: 20.0 g/L
• Sodium Chloride: 5.0 g/L
• Lactose: 5.0 g/L
• Dipotassium Phosphate: 2.75 g/L
• Potassium Dihydrogen Phosphate: 2.75 g/L
• Sodium Lauryl Sulfate: 0.1 g/L
• MUG (4-Methylumbelliferyl-β-D-glucuronide): 0.1 g/L

This unique formulation results in a final pH of 7.4 ± 0.1 at 25°C, crucial for the optimal growth of E. coli O157. Adjustments can be made as necessary to meet specific performance needs.

Directions for Use

To utilize the mE.Coli Broth, follow these simple steps:

1. Suspend: Mix 37.0 g of the granules in 1 liter of distilled water.
2. Dispense: Transfer into 225 mL flasks.
3. Autoclave: Sterilize at 121°C for 15 minutes.
4. Cool: Allow the broth to cool down to room temperature.
5. Supplement: Add a vial of sterilized Novobiocin (4.5 mg) to achieve a final concentration of 20 µg/mL.
6. Mix: Ensure even distribution of Novobiocin.
7. Inoculate: After adding your sample solution, incubate the mixture at 42°C for 18-24 hours in a water bath.

These steps will help you achieve effective selective enrichment, allowing for reliable isolation of E. coli O157 in various samples.

Benefits of Using mE.Coli Broth

Utilizing mE.Coli Broth (mEC+n) in your lab not only promotes the growth of E. coli O157 but also supports precise microbial analysis. This broth is crafted to enhance selectivity, reducing the risk of false positives during your testing process. The inclusion of sodium lauryl sulfate further aids in inhibiting unwanted bacterial flora, ensuring that you obtain the most accurate results possible.

Additionally, the broth’s composition fosters a conducive environment for E. coli O157, improving recovery rates from food and clinical samples. Laboratories focusing on food safety, environmental monitoring, and clinical diagnostics will find this product invaluable.

Conclusion

In conclusion, the mE.Coli Broth (mEC+n) is a critical component for any laboratory engaged in the detection and analysis of E. coli O157. Its optimized formulation and straightforward preparation process make it a top choice for microbiological testing. By incorporating this selective enrichment broth into your protocols, you ensure high-quality results and improved reliability in your analyses.