Description

mCPC Medium for Vibrio Isolation – FDA Compliant Culture Medium with Babio-4151 Additives

Product Overview

Discover the mCPC Medium, a specialized culture medium designed for the effective isolation of Vibrio species, including Vibrio vulnificus, adhering to FDA standards. This high-performance medium is enriched with essential nutrients and inhibitors, making it the go-to solution for microbiology labs focused on aquatic pathogens.

Key Features

• FDA-Standard Formulation: Compliant with regulatory standards for safe and reliable testing.
• Efficient Isolation of Vibrio: Specifically tailored for isolating pathogenic Vibrio species, crucial for food safety and aquatic health studies.
• Nutrient-Rich Composition: Contains pancreatic digest of casein, beef extract powder, and sodium chloride to provide a robust growth environment.
• Effective Inhibitors: Polymyxin B and E effectively inhibit non-target bacteria such as Vibrio parahaemolyticus, ensuring selective growth.
• Easy to Use: Quick preparation process—just mix with distilled water and additives, then pour into plates without the need for autoclaving.
• pH Monitoring: Utilizes bromothymol blue and cresol red as pH indicators to monitor bacterial fermentation and acid production.

Ingredients (gm/liter)

• Pancreatic Digest of Casein: 10.0
• Sodium Chloride: 20.0
• Agar: 15.0
• Bromothymol Blue: 0.04
• Cresol Red: 0.04
• Beef Extract Powder: 5.0

Final pH: 7.5-7.7 at 25℃

Preparation Instructions

1. Suspend 5.0 g of mCPC Medium in 90 mL of distilled water.
2. Heat to boiling and dissolve completely.
3. Cool to 48-55℃.
4. Add 10 mL of HB4151b 10% Cellobiose Solution and one HB4151a mCPC Supplement.
5. Mix thoroughly and pour into plates—no autoclaving required.

Principle and Interpretation

The mCPC Medium provides a comprehensive nutrient base, essential for the growth of Vibrio species. Peptones and beef extract supply vital carbon and nitrogen sources, while sodium chloride maintains osmotic balance. Cellobiose serves as a fermentable sugar, enabling Vibrio vulnificus to produce acid and change the medium’s color to yellow, aiding in visual identification.

Precautions

• For laboratory use only.
• Do not use dried medium that is past its shelf life, shows signs of caking, or has color variations.

Storage Conditions and Shelf Life

• Storage: Keep tightly capped in the original container at 5-30°C.
• Shelf Life: Dried medium lasts for 3 years from the manufacturing date. Prepared medium should be stored at 2-8°C and shielded from direct light.

Why Choose mCPC Medium?

Utilize mCPC Medium for your laboratory’s Vibrio isolation needs. With its FDA compliance, efficient formulation, and easy preparation, it’s an essential tool for researchers and microbiologists focused on aquatic pathogens, food safety, and public health. Ensure accurate results in your microbial analysis today!

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