Mannitol Salt Agar Medium

Description

Mannitol Salt Agar Medium for Selective Isolation of Staphylococcus aureus

Mannitol Salt Agar Medium enables the selective isolation of Staphylococcus aureus, making it valuable for clinical labs and research. Its high sodium chloride concentration (75 g/L) supports S. aureus growth while preventing most other bacteria from growing. This medium is ideal for clinical diagnostics and laboratory testing, allowing users to differentiate S. aureus from other microorganisms.

Key Ingredients and Their Functions

Mannitol Salt Agar Medium uses carefully chosen ingredients to promote Staphylococcus aureus growth:

• Pancreatic Digest of Casein (5.0 g/L) and Peptic Digest of Animal Tissue (5.0 g/L): These provide essential nutrients like nitrogen, carbon, and amino acids.

• Beef Extract (1.0 g/L): This ingredient supplies growth factors and vitamins.

• D-Mannitol (10.0 g/L): It aids in differentiating S. aureus through fermentation, leading to a visible color change.

• Sodium Chloride (75.0 g/L): This high salt content blocks non-halophilic bacteria, ensuring only S. aureus thrives.

• Agar (15.0 g/L): It solidifies the medium, creating a surface for colony growth.

• Phenol Red (0.025 g/L): This pH indicator shifts color when bacteria ferment mannitol, aiding identification.

The medium maintains a final pH of 7.4 ± 0.2, which promotes optimal microbial growth.

Preparation Steps

To prepare, mix 112.5 g of powder with 1 liter of distilled or deionized water. Stir until dissolved. Autoclave the solution at 121°C for 15 minutes. Let it cool before pouring into sterile Petri dishes. After solidification, the medium is ready for isolating S. aureus.

Applications in Microbiology

Mannitol Salt Agar Medium suits various microbiology fields, supporting clinical and industrial uses:

• Clinical Diagnostics: It detects S. aureus infections, aiding the identification of bacteria responsible for skin infections or pneumonia.

• Food Safety Testing: Labs use it to find S. aureus contamination in food, ensuring consumer safety.

• Pharmaceutical Testing: It checks for microbial contamination in pharmaceuticals, ensuring product quality.

Benefits of Mannitol Salt Agar Medium

1. Efficient Selective Isolation: The high salt content supports S. aureus growth while inhibiting other bacteria.

2. Easy Differentiation: With mannitol and phenol red, users quickly identify S. aureus through color changes.

3. Versatile Use: It fits clinical labs, food safety testing, and pharmaceutical research, providing reliable results.

4. Quick Preparation: Its easy preparation saves time for labs needing fast, accurate testing. visit our [United States Pharmacopeia] for more related products