Description
Overview
M-Endo Agar LES is a professional culture medium developed for accurate coliform testing in water by membrane filtration. It supports rapid detection of total coliforms and lactose-fermenting bacteria in drinking water, industrial water, and environmental samples.
Because water safety matters, laboratories need a reliable solution that delivers clear colony differentiation. This medium provides strong selectivity while preserving excellent recovery rates. As a result, microbiology labs can work faster and with higher confidence.
Key Benefits
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Designed for membrane filtration method
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High selectivity against Gram-positive bacteria
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Clear red colonies for lactose fermenters
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Excellent performance in water microbiology
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Suitable for drinking water and wastewater analysis
Moreover, the balanced nutrient system promotes stable growth. Therefore, technicians can achieve consistent and repeatable results across batches.
Composition & Performance
The formula combines yeast extract, tryptose, peptones, and casein hydrolysate to supply nitrogen, vitamins, and minerals. Lactose serves as the fermentable carbohydrate. Sodium chloride maintains osmotic balance.
Meanwhile, dipotassium phosphate and monopotassium phosphate stabilize pH. Deoxycholate and SDS inhibit Gram-positive organisms. Basic fuchsin works as an indicator, while sodium sulfite reduces background color.
When coliform bacteria ferment lactose, aldehydes react with sulfite and fuchsin. Consequently, red colonies appear on a light background. This contrast simplifies identification during routine coliform testing.
Applications
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Drinking water quality control
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Environmental water monitoring
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Wastewater analysis
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Public health laboratories
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Food and beverage water systems
In addition, this medium integrates seamlessly into standard membrane filtration workflows. Labs can adopt it without changing existing protocols.
Preparation Guide
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Weigh 51.0 g of powder.
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Add 20 ml of 95% ethanol.
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Mix with 980 ml of distilled water.
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Heat with stirring until fully dissolved.
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Dispense and autoclave at 121°C for 15 minutes.
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Cool to 45–50°C.
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Pour into sterile Petri dishes (50–60 mm).
This simple process saves time while ensuring consistent quality.
Why Choose This Medium
M-Endo Agar LES delivers precision in coliform detection. It supports regulatory testing standards and daily laboratory work. Furthermore, it reduces false positives and improves reading clarity.
Because every water test influences public safety, laboratories need dependable performance. This medium helps teams act quickly, interpret results easily, and maintain compliance with water microbiology protocols.
Used worldwide in membrane filtration workflows, it stands as a trusted choice for water testing professionals. By integrating this solution, laboratories strengthen both efficiency and data integrity.
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