Lysine Decarboxylase Broth for Microbial Identification

Description

Product Description for Lysine Decarboxylase Broth

Overview

Lysine Decarboxylase Broth is a specialized culture medium designed for the identification of microorganisms, particularly enteric bacilli. This broth enables the detection of lysine decarboxylation, a key metabolic process utilized in differentiating bacterial species.

Key Ingredients

• Gelatin Peptone: 5.0 g/L
• Yeast Extract: 3.0 g/L
• Dextrose: 1.0 g/L
• L-Lysine: 5.0 g/L
• Bromcresol Purple: 0.02 g/L
• Final pH: 6.8 ± 0.2 at 25°C

Preparation Instructions

1. Suspension: Dissolve 14.0 g of Lysine Decarboxylase Broth in one liter of deionized or distilled water.
2. Distribution: Pour the prepared medium into fermentation tubes (Durham) to allow for gas collection.
3. Sterilization: Autoclave at 121°C for 15 minutes to eliminate contaminants and ensure a sterile environment.

Application

Lysine Decarboxylase Broth is widely utilized in microbiological laboratories for:

• Microbial Identification: Especially effective for enteric bacilli.
• Metabolic Studies: Facilitates the study of lysine metabolism in various bacterial species.

Features

• Reliable Performance: Supports accurate differentiation of gram-negative bacteria based on lysine decarboxylation.
• Visual pH Indicator: Bromcresol purple changes color, providing immediate feedback on microbial activity.
• Ease of Use: Simple preparation and sterilization process make it ideal for routine laboratory use.

Benefits

Incorporating Lysine Decarboxylase Broth into laboratory protocols enhances microbial identification processes. Its reliable formulation and straightforward preparation make it essential for accurate microbiological testing. By promoting lysine decarboxylation, this broth aids in differentiating enteric bacilli from other bacteria, ensuring precise analysis in both clinical and research environments.

Conclusion

Utilize Lysine Decarboxylase Broth to elevate your microbial identification capabilities. Its effective ingredients and user-friendly preparation establish it as a preferred medium for microbiologists focused on enteric bacilli and related microorganisms. For any laboratory engaged in microbial studies, this broth is an invaluable tool, ensuring reliable results in your research endeavors.

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