Description
LST Medium
Introducing our Lauryl Sulfate Tryptose Broth LST Medium, specifically designed for the detection of coliform bacteria and Escherichia coli. This high-quality Laurel Sulfate Tryptone Broth comes in a convenient 10ml plastic tube, with each unit individually packaged to ensure maximum protection against leakage and contamination. Each box contains 20 tubes, making it an ideal choice for laboratory settings.
The LST Medium supports the growth of targeted bacterial strains by providing essential nutrients. The inclusion of peptone serves as a vital carbon and nitrogen source, while sodium chloride maintains a balanced osmotic pressure. Lactose acts as a fermentable carbohydrate, promoting the growth of coliforms, while potassium dihydrogen phosphate and dipotassium phosphate function as effective buffering agents. Notably, sodium lauryl sulfate inhibits the growth of non-coliform bacteria, ensuring accurate results.
Quality Control Strains
For optimal results, we recommend inoculating the Lauryl Sulfate Tryptose Broth LST Medium with the following quality control strains, maintaining a temperature of 36±1℃ for 24-48 hours. The table below summarizes the strains and expected outcomes:
| Quality Control Strain | Strain Number | Inoculum Amount (CFU) | Reference Medium | Method | Expected Result |
|---|---|---|---|---|---|
| Salmonella typhimurium | ATCC 14028 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2, no gas production |
| Citrobacter freundii | ATCC 43864 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2, gas fills 1/3 of tube |
| Enterococcus faecalis | ATCC 29212 | 1000 – 5000 | TSA | Semi-quantitative | Turbidity 0 (no growth) |
| Escherichia coli | ATCC 8739 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2, gas fills 1/3 of tube |
Why Choose LST Medium?
Selecting the right medium is crucial for accurate microbiological analysis. Our LST Medium not only meets the stringent requirements for detecting coliforms but also aligns with current laboratory best practices. The formulation is optimized for sensitivity, ensuring reliable results in identifying and quantifying bacterial populations. This product is essential for laboratories focusing on food safety, water quality testing, and clinical diagnostics.
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