Description

Selective Enrichment Broth for Enterobacter sakazakii

Overview

Explore our Modified Lauryl Sulfate Tryptose Broth, specifically formulated for the selective enrichment culture of Enterobacter sakazakii. This nutrient-rich broth is an essential tool in microbiological analysis, particularly for laboratories focusing on food safety and pathogen detection. With the inclusion of vancomycin, this medium enhances the isolation of Enterobacter sakazakii, ensuring that your results are both reliable and reproducible.

Key Components

Our formulation combines a variety of high-quality components to support the optimal growth of Enterobacter sakazakii:

• Pancreatic Digest of Casein (20.0 g/L): Provides essential amino acids and peptides.
• Sodium Chloride (34.0 g/L): Maintains osmotic balance.
• Lactose (5.0 g/L): Serves as a fermentable carbohydrate source.
• Dipotassium Hydrogen Phosphate (2.75 g/L) and Potassium Dihydrogen Phosphate (2.75 g/L): Buffering agents that help maintain pH stability.
• Sodium Lauryl Sulfate (0.1 g/L): A surfactant that aids in the selective growth of target organisms.

Preparation Instructions

To prepare the broth, follow these straightforward steps:

1. Suspend 64.6 g of the powdered medium in 1 liter of distilled water.
2. Dispense the mixture into 100 mL flasks.
3. Autoclave at 121 ℃ for 15 minutes to sterilize.
4. Cool the flasks to 45-50 ℃ and add 1 mg of filter-sterilized vancomycin (1 mg/mL) solution.
5. Transfer 10 mL of the enriched broth into sterile test tubes and mix thoroughly.

This simple preparation method ensures that your broth is ready for use in enriching Enterobacter sakazakii cultures, adhering to GB standards for laboratory practices.

Optimal Conditions

The broth maintains a pH of 6.8 ± 0.2 at 25 ℃, creating an ideal environment for the growth of Enterobacter sakazakii. The addition of vancomycin acts effectively against Gram-positive bacteria, allowing for the selective enrichment of your targets without interference.

Applications

This selective enrichment broth is particularly valuable in food microbiology, clinical diagnostics, and environmental testing. By utilizing our Modified Lauryl Sulfate Tryptose Broth, you can enhance your laboratory’s efficiency in detecting Enterobacter sakazakii, a known pathogen associated with serious health risks, especially in infants.

Conclusion

Incorporating our Modified Lauryl Sulfate Tryptose Broth into your laboratory workflow not only streamlines the process of enriching Enterobacter sakazakii but also enhances the reliability of your microbiological analyses. With its carefully balanced components and straightforward preparation, this broth stands out in the market.

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