Description
Double Lactose Peptone Broth: A Comprehensive Guide
Product Overview
The Double Lactose Peptone Broth detects and enumerates coliforms and Escherichia coli. It comes in 10ml plastic tubes, with 20 tubes per box, making handling easy. Each tube remains sealed to prevent leakage, ensuring reliable microbial testing.
Key Features
Formulation
This broth features peptones and beef extract for essential nutrients. Lactose serves as a fermentable sugar, while sodium chloride maintains osmotic balance. Additionally, bromocresol purple acts as a pH indicator, changing color in acidic conditions.
Quality Control Results
In quality control tests, Escherichia coli shows turbidity and gas production. The medium turns yellow due to acid production. Other strains like Salmonella typhimurium and Staphylococcus aureus also show turbidity, confirming the medium’s effectiveness.
Microbial Sensitivity Testing
For optimal results, inoculate the broth with quality control strains and incubate at 36±1°C for 24±2 hours. The following summarizes the results:
| Quality Control Strain | Strain Number | Inoculum (CFU) | Comparative Counting Medium | Method | Quality Control Result | Other Characteristics |
|---|---|---|---|---|---|---|
| Escherichia coli | ATCC25922 | 10-100 | TSA | Semi-quantitative | Turbidity 2, and gas fills 1/3 of the inverted vial | Turns acid, yellow color |
| Salmonella typhimurium | ATCC14028 | 10-100 | TSA | Semi-quantitative | Turbidity 2 | / |
| Staphylococcus aureus | ATCC6538 | 10-100 | TSA | Semi-quantitative | Turbidity 2 | Turns acid, yellow color |
| Citrobacter freundii | ATCC43864 | 10-100 | TSA | Semi-quantitative | Turbidity 2, and gas fills 1/3 of the inverted vial | Turns acid, yellow color |
Applications
Researchers widely use Double Lactose Peptone Broth in microbiology labs to test water quality and food safety. This broth helps identify fecal contamination, which ensures public health safety. Its formulation supports rapid growth of coliform bacteria, making it essential for accurate results.
Conclusion
In summary, the Double Lactose Peptone Broth provides a reliable medium for detecting coliforms and E. coli. With its balanced formulation and proven performance, this broth meets the demands of modern microbiological testing. Enhance your lab’s microbial detection capabilities with Double Lactose Peptone Broth! visit our [Ready-to-use culture medium] for more related products
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