Description
Lactose Peptone Broth Tubes: Your Essential Microbiological Culture Medium
Lactose Peptone Broth Tubes is a high-quality microbiological culture medium. It is specifically designed for determining coliforms and Escherichia coli in various samples. Each pack contains 20 sterile 10ml plastic tubes. These tubes are individually packaged to prevent contamination and leakage. This product meets strict laboratory testing requirements. Thus, it becomes an indispensable tool for microbiologists.
Key Features and Benefits
Lactose Peptone Broth combines essential nutrients to support the growth of target microorganisms. The peptones and beef extract provide vital carbon and nitrogen sources. Additionally, lactose serves as a fermentable carbohydrate. This formulation promotes robust microbial growth and detects acid and gas production. These factors are crucial for identifying bacterial species like E. coli.
Moreover, sodium chloride maintains osmotic balance. This balance is important for the integrity of microbial cells during cultivation. Bromocresol purple acts as a pH indicator. It turns yellow in acidic conditions when lactose ferments. Therefore, this feature allows for quick visual assessments of microbial activity.
Testing and Quality Control
To ensure quality, Lactose Peptone Broth undergoes stringent testing with established quality control strains. Specifically, researchers inoculate Escherichia coli (BBATCC 25922), Salmonella typhimurium (BBATCC 14028), Staphylococcus aureus (BBATCC 6538), and Citrobacter freundii (BBATCC 43864) at concentrations of 10-100 CFU. Then, they incubate the culture at 36±1°C for 24±2 hours. Successful fermentation results in turbidity of 2, gas filling one-third of the tube, and a color change to yellow. This indicates the presence of acid.
| Quality control strain | Strain number | Inoculum (CFU) | Ratio or counting culture medium | Method | Quality control result | Other characteristics |
|---|---|---|---|---|---|---|
| Escherichia coli | BB ATCC25922 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2, and gas fills 1/3 of the tube. | Turns acid and yellow. |
| Salmonella typhimurium | BB ATCC14028 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | |
| Staphylococcus aureus | BB ATCC6538 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | Turns acid and yellow. |
| Citrobacter freundii | BB ATCC43864 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2, and gas fills 1/3 of the tube. | Turns acid and yellow. |
Application and Usage
Lactose Peptone Broth is ideal for clinical laboratories, food testing, and environmental microbiology. It effectively detects coliform bacteria, an important indicator of water quality and food safety. Microbiologists can rely on this culture medium for accurate and reliable results in their research and testing.
Conclusion
In summary, Lactose Peptone Broth is a versatile and reliable culture medium. It supports the growth of critical microorganisms, facilitating effective detection and analysis. Its ease of use, combined with quality control measures, makes it a preferred choice in microbiological studies. Choose Lactose Peptone Broth for your laboratory needs. This choice ensures accurate results in your microbial testing today! visit our [Ready-to-use culture medium] for more related products
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