Lactose Peptone Broth Granule

Description

Lactose Peptone Broth Granule: Product Description for Water Testing

Lactose Peptone Broth Granule is ideal for detecting total coliforms in drinking water and water sources. This medium supports the growth of coliform bacteria, commonly used to identify contamination in water supplies. Water testing labs rely on this broth to ensure the safety of water by detecting harmful bacteria like coliforms.

Composition and Key Ingredients

The formulation contains specific ingredients that promote the growth of total coliforms:

• Peptic digest of animal tissue (10.0 g/L) provides nutrients for bacterial growth.
• Beef extract (3.0 g/L) supplies vitamins and essential growth factors.
• Lactose (5.0 g/L) enables bacteria to produce gas by fermenting the sugar.
• Sodium chloride (5.0 g/L) helps maintain the osmotic balance.
• Bromocresol purple (0.016 g/L) is a pH indicator that changes color with fermentation.
• Final pH: 7.3-7.5 at 25°C ensures optimal growth conditions for coliform bacteria.

These ingredients ensure the broth is effective in detecting coliforms quickly and accurately.

Easy Preparation Instructions

To prepare Lactose Peptone Broth Granule, follow these simple steps:

1. Dissolve 2.3 g in 100 ml of distilled water.
2. Transfer 10 ml into tubes containing an inverted fermentation tube.
3. Autoclave at 115°C for 20 minutes to sterilize.

Once prepared, the broth is ready for use. Coliform bacteria ferment the lactose and produce gas, which is visible in the fermentation tube, indicating contamination.

Water Testing Applications

Lactose Peptone Broth Granule is widely used in drinking water testing. It helps detect harmful bacteria that may contaminate water supplies. Detecting total coliforms ensures that the water remains safe for consumption. The broth is also used to test other water sources like rivers, lakes, and sewage, ensuring environmental safety.

Total coliform detection in drinking water is critical for maintaining public health standards. This broth provides consistent and accurate results in water quality testing.

Quality and Storage

To preserve its quality, store Lactose Peptone Broth in a dark and dry place. Proper storage prevents the medium from degrading and ensures reliable results. Regular quality checks help maintain consistent performance in coliform detection.

Conclusion

In conclusion, Lactose Peptone Broth Granule is a trusted medium for detecting total coliforms in drinking water and other water sources. It’s simple to prepare, reliable, and essential for maintaining water safety. Labs use it for routine water testing, ensuring that water remains free of harmful bacteria.