Description
Lactose Peptone Broth (Triple Strength) – 5ml for Microbial Culture
Lactose Peptone Broth, often referred to as Lactose Peptone Medium, is a reliable solution for microbial culture and identification. Designed for bacterial analysis, it is ideal for laboratories that focus on microbial testing. Packaged in 5ml sterile plastic tubes, each unit is individually sealed to prevent contamination. The leak-proof design ensures safe handling during transport and storage, making it highly convenient for laboratory use.
Product Composition and Function
Lactose Peptone Broth contains a balanced mix of peptone, beef extract, lactose, sodium chloride, and bromocresol purple (as a pH indicator). Peptone and beef extract provide essential nutrients like carbon and nitrogen, supporting bacterial growth. Lactose serves as the fermentable sugar that differentiates lactose-fermenting bacteria. Sodium chloride helps maintain osmotic balance, while bromocresol purple indicates pH changes. When bacteria produce acid, the broth turns yellow, and in alkaline conditions, it remains purple. This color change helps identify bacterial activity and fermentation.
Key Bacterial Results
In quality control testing, various bacterial strains show specific growth patterns in Lactose Peptone Broth:
- Escherichia coli (BBATCC 25922): Turbidity of 2, gas fills 1/3 of the tube, and the broth turns yellow.
- Salmonella typhimurium (BBATCC 14028): Turbidity of 2, no gas, and no color change.
- Staphylococcus aureus (BBATCC 25923): Turbidity of 2, no gas, but the broth turns yellow.
- Enterobacter cloacae (BBATCC 23355): Turbidity of 2, gas fills 1/3 of the tube, and the broth turns yellow.
Microbial Sensitivity Test
This Lactose Peptone Broth undergoes rigorous microbial sensitivity testing. When tested with 10-100 colony-forming units (CFU) of the following strains, results show distinct patterns:
| Strain | Strain Number | CFU | Growth | Result |
|---|---|---|---|---|
| Salmonella typhimurium | BBATCC 14028 | 10-100 | +++ | No gas, no yellowing |
| Escherichia coli | BBATCC 25922 | 10-100 | +++ | Gas, yellowing |
| Staphylococcus aureus | BBATCC 25923 | 10-100 | +++ | No gas, yellowing |
| Enterobacter cloacae | BBATCC 23355 | 10-100 | +++ | Gas, yellowing |
Highly Effective for Microbial Identification
Lactose Peptone Broth efficiently detects lactose-fermenting bacteria like E. coli. Its clear differentiation between fermenters and non-fermenters, such as Salmonella, ensures accurate microbial identification. The triple-strength formula enhances sensitivity for even small inoculum samples, providing reliable results. It is widely used in applications like food safety testing, water analysis, and clinical diagnostics. visit our [Ready-to-use culture medium] for more related products
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