Description
Lactose Bile Ferment Broth – 10ml Tubes for Accurate E. coli Testing
Overview
Lactose Bile Ferment Broth is a vital medium specifically designed for determining coliform bacteria, especially Escherichia coli. Each box includes 20 individually packaged 10ml tubes, ensuring that you maintain a sterile environment. Moreover, the innovative design features a small inverted tube that minimizes leakage and safeguards the sample integrity throughout the testing process.
Composition and Functionality
This specialized culture medium consists of peptone, which provides essential carbon and nitrogen sources that effectively support bacterial growth. Additionally, bile salts work to inhibit the growth of Gram-positive bacteria. Consequently, this inhibition ensures that only target organisms, such as E. coli, proliferate. The primary fermentable sugar, lactose, allows for precise identification, as it is specifically utilized by coliform bacteria. Furthermore, the addition of bromocresol purple serves as a pH indicator; thus, the medium changes color to yellow in acidic conditions and remains purple in alkaline conditions. This color change facilitates easy visual confirmation of fermentation activity.
Microbial Sensitivity Testing
To validate the effectiveness of the Lactose Bile Ferment Broth, you can perform inoculation tests with quality control strains. Specifically, these tests involve incubating the tubes at 36±1°C for 24±2 hours in an aerobic environment. The following strains have been tested, demonstrating consistent results:
| Control Strains | Strain Numbers | Inoculum Size (CFU) | Growth Situation | Results |
|---|---|---|---|---|
| Escherichia coli | BBATCC 25922 | 10-100 | ++ | Acid and gas production |
| Salmonella typhimurium | BBATCC 14028 | 10-100 | ++ | Acid and no gas production |
| Enterococcus spp. | BBATCC 29212 | 1000-5000 | – | Inhibited |
| Citrobacter freundii | BBATCC 43864 | 10-100 | ++ | Acid and gas production |
| Enterobacter cloacae | BBATCC 33355 | 10-100 | ++ | Acid and gas production |
| Aeromonas hydrophila | BBATCC 13048 | 10-100 | ++ | Acid and gas production |
Applications
Lactose Bile Ferment Broth is widely used in microbiological laboratories for E. coli detection and coliform enumeration in food, water, and clinical samples. Consequently, this medium ensures accurate and reliable results, making it an invaluable tool for food safety testing and quality control.
Conclusion
When you choose Lactose Bile Ferment Broth, you are opting for precision in coliform detection. The robust formulation, combined with effective inhibition of non-target bacteria, enhances the reliability of your microbial testing. Therefore, whether for research or routine quality control, this broth meets the rigorous standards of modern microbiology.
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